Campos-Pires Rita, Yonis Amina, Macdonald Warren, Harris Katie, Edge Christopher J, Mahoney Peter F, Dickinson Robert
Anaesthetics, Pain Medicine and Intensive Care Section, Department of Surgery and Cancer, Imperial College London; Royal British Legion Centre for Blast Injury Studies, Department of Bioengineering, Imperial College London.
Anaesthetics, Pain Medicine and Intensive Care Section, Department of Surgery and Cancer, Imperial College London.
J Vis Exp. 2018 Dec 21(142). doi: 10.3791/58400.
Traumatic brain injury is a leading cause of death and disability in military and civilian populations. Blast traumatic brain injury results from the detonation of explosive devices, however, the mechanisms that underlie the brain damage resulting from blast overpressure exposure are not entirely understood and are believed to be unique to this type of brain injury. Preclinical models are crucial tools that contribute to better understand blast-induced brain injury. A novel in vitro blast TBI model was developed using an open-ended shock tube to simulate real-life open-field blast waves modelled by the Friedlander waveform. C57BL/6N mouse organotypic hippocampal slice cultures were exposed to single shock waves and the development of injury was characterized up to 72 h using propidium iodide, a well-established fluorescent marker of cell damage that only penetrates cells with compromised cellular membranes. Propidium iodide fluorescence was significantly higher in the slices exposed to a blast wave when compared with sham slices throughout the duration of the protocol. The brain tissue injury is very reproducible and proportional to the peak overpressure of the shock wave applied.
创伤性脑损伤是导致军人和平民死亡及残疾的主要原因。爆炸所致创伤性脑损伤由爆炸装置引爆引起，然而，爆炸超压暴露导致脑损伤的潜在机制尚未完全明确，且被认为是此类脑损伤所特有的。临床前模型是有助于更好地理解爆炸所致脑损伤的关键工具。利用开放式激波管开发了一种新型体外爆炸创伤性脑损伤模型，以模拟由弗里德兰德波形建模的真实野外爆炸波。将C57BL/6N小鼠海马脑片培养物暴露于单次冲击波下，并使用碘化丙啶（一种成熟的细胞损伤荧光标记物，仅穿透细胞膜受损的细胞）对长达72小时的损伤发展情况进行表征。在整个实验过程中，与假手术切片相比，暴露于冲击波的切片中碘化丙啶荧光显著更高。脑组织损伤具有高度可重复性，且与所施加冲击波的峰值超压成正比。
