Borrero E, Rosenthal D, Otis J B
Department of Vascular Surgery, Georgia Baptist Medical Center, Atlanta.
Ann Vasc Surg. 1988 Jan;2(1):69-72. doi: 10.1016/S0890-5096(06)60780-2.
Use of the Neodymium: yttrium -aluminum -garnet (Nd:YAG) laser to recanalize stenosed arteries may require delivery of the beam through blood. To assess the degree of hemolysis and debris formation, 54 samples of citrated whole blood were exposed to Nd:YAG laser radiation of varying powers (10, 20 and 30 watts) and duration (1, 2.5 and 5 seconds). Compared to control samples which were not subjected to laser light, there was no significant decrease in hematocrit (41 to 40.5 +/- 5%), hemoglobin concentration (13.8 to 13.8 +/- .06 g/1OO ml), or increase in "free" hemoglobin concentration. Debris weight (from .45 +/- .002 to .45 +/- .002 mg), as well as the white blood cell count, was also not significantly changed (from 5,400 to 5,200 +/- 240 WBC/cm). Light microscopy examination of debris from samples of whole blood, washed erythrocytes, and platelet-rich plasma subjected to the laser at 30 watts for five seconds failed to demonstrate the presence of membrane denaturation of blood elements, as compared with the morphologic changes observed in whole blood samples exposed to a "hot tip" rather than Nd:YAG laser radiation. Nd:YAG laser can be used intravascularly without fear of hemolysis or debris "micro-embolization" up to a power of 30 watts for five seconds.
使用钕钇铝石榴石（Nd:YAG）激光使狭窄动脉再通可能需要让光束穿过血液。为评估溶血程度和碎片形成情况，将54份枸橼酸盐抗凝全血样本暴露于不同功率（10瓦、20瓦和30瓦）和持续时间（1秒、2.5秒和5秒）的Nd:YAG激光辐射下。与未接受激光照射的对照样本相比，血细胞比容（从41降至40.5 +/- 5%）、血红蛋白浓度（从13.8降至13.8 +/- 0.06 g/100 ml）没有显著降低，“游离”血红蛋白浓度也没有升高。碎片重量（从0.45 +/- 0.002毫克降至0.45 +/- 0.002毫克）以及白细胞计数（从5400降至5200 +/- 240个白细胞/cm）也没有显著变化。对全血样本、洗涤过的红细胞样本和富含血小板血浆样本在30瓦功率下照射5秒后产生的碎片进行光学显微镜检查，与暴露于“热尖端”而非Nd:YAG激光辐射的全血样本中观察到的形态学变化相比，未发现血液成分的膜变性。Nd:YAG激光在血管内使用时，功率达30瓦持续5秒也不用担心溶血或碎片“微栓塞”。
