Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE; Shandong Key Laboratory of Biochemical Analysis; Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong; College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P.R. China.
Anal Chem. 2020 May 5;92(9):6734-6740. doi: 10.1021/acs.analchem.0c00849. Epub 2020 Apr 23.
A new photoelectrochemical (PEC) "signal-on" sensing platform based on photoactive material BiO-ZnO and CdS quantum dots (QDs) sensitizer was fabricated for ultrasensitive determination of thrombin by constructing supersandwich nanowires. The CdS/ZnO/BiO sensitization structure with excellent energy level arrangement remarkably improved photoelectric conversion efficiency because of the efficient separation of the electron-hole. Moreover, the DNA supersandwich nanowire is ingeniously synthesized in one step by simple dislocation hybridization, which could carry a large amount of sensitized material CdS QDs. More importantly, with Exonuclease III (Exo III)-assisted multiple amplification, the proposed "signal-on" platform demonstrated a detection range of 10 fM to 1 μM with the detection limit of 1.41 fM for thrombin. Impressively, the PEC platform can successfully detect human serum samples with good accuracy. Above all, the CdS/ZnO/BiO sensitization photoelectric biosensing platform by using DNA nanowire in combination with Exo III-multiple amplification opens new sensitized amplification paths for supersensitive biosensing and bioanalysis.
基于光活性材料 BiO-ZnO 和 CdS 量子点 (QD) 敏化剂的新型光电化学 (PEC)“信号开启”传感平台，通过构建超三明治纳米线，用于超灵敏测定凝血酶。CdS/ZnO/BiO 敏化结构具有优异的能级排列，由于载流子的有效分离，显著提高了光电转换效率。此外，通过简单的位错杂交一步巧妙合成 DNA 超三明治纳米线，可以携带大量敏化材料 CdS QDs。更重要的是，通过 Exonuclease III (Exo III)-辅助的多重扩增，所提出的“信号开启”平台对凝血酶的检测范围为 10 fM 至 1 μM，检测限低至 1.41 fM。令人印象深刻的是，PEC 平台可以成功地以良好的准确度检测人血清样本。总之，CdS/ZnO/BiO 敏化光电生物传感平台结合使用 DNA 纳米线和 Exo III-多重扩增，为超灵敏生物传感和生物分析开辟了新的敏化放大途径。
