Theoretical Biophysics, Humboldt-Universität zu Berlin, Berlin, Germany.
In Silico Biol. 2020;14(1-2):71-83. doi: 10.3233/ISB-190473.
Moonlighting refers to a protein with at least two unrelated, mechanistically different functions. As a concept, moonlighting describes a large and diverse group of proteins which have been discovered in a multitude of organisms. As of today, a systematized view on these proteins is missing. Here, we propose a classification of moonlighting proteins by two classifiers. We use the function of the protein as a first classifier: activating - activating (Type I), activating - inhibiting (Type II), inhibiting - activating (Type III) and inhibiting - inhibiting (Type IV). To further specify the type of moonlighting protein, we used a second classifier based on the character of the factor that switches the function of the protein: external factor affecting the protein (Type A), change in the first pathway (Type B), change in the second pathway (Type C), equal competition between both pathways (Type D). Using a small two-pathway model we simulated these types of moonlighting proteins to elucidate possible behaviors of the types of moonlighting proteins. We find that, using the results of our simulations, we can classify the behavior of the moonlighting types into Blinker, Splitter andSwitch.
双重功能蛋白是指至少具有两种不相关的、在机制上不同的功能的蛋白。作为一个概念，双重功能蛋白描述了在多种生物体中发现的一大组多样化的蛋白。截至目前，对这些蛋白还缺乏系统的认识。在这里，我们提出了一种通过两个分类器对双重功能蛋白进行分类的方法。我们首先根据蛋白的功能将其分类：激活-激活（I 型）、激活-抑制（II 型）、抑制-激活（III 型）和抑制-抑制（IV 型）。为了进一步确定双重功能蛋白的类型，我们使用了第二个基于切换蛋白功能的因子特征的分类器：影响蛋白的外部因子（A 型）、第一途径的变化（B 型）、第二途径的变化（C 型）、两条途径之间的平等竞争（D 型）。使用一个小型的双途径模型，我们模拟了这些类型的双重功能蛋白，以阐明双重功能蛋白类型的可能行为。我们发现，通过模拟结果，我们可以将双重功能蛋白的行为分类为闪烁器、分裂器和开关。
