Institute of Industrial Biotechnology, Government College University, Lahore 54000, Pakistan.
School of Biological Sciences, University of The Punjab, Lahore 54590, Pakistan.
Future Microbiol. 2020 Mar;15:333-348. doi: 10.2217/fmb-2019-0158.
The objective of this study was to identify the genes involved in plantaricin synthesis and adaptive stress response in four strains (AS-6, AS-8, AS-9 and AS-10) and one strain (AS-7) for their usage in medicine and industry. Whole genomes of these strains were sequenced by a high-throughput sequencing technique known as next-generation sequencing via Ilumina MiSeq platform and the genes were identified by using various bioinformatics tools and software. Plantaricin genes (, , , , ) and genes regulating response to temperature, pH, bile salt, osmotic and oxidative stress were identified in all strains. Lactobacilli could be an option to combat antimicrobial resistance and might replace harmful antibiotics in future.
本研究的目的是鉴定四个菌株（AS-6、AS-8、AS-9 和 AS-10）和一个菌株（AS-7）中与乳杆菌素合成和适应应激反应相关的基因，以便在医学和工业中使用。这些菌株的全基因组通过高通量测序技术（称为 Illumina MiSeq 平台的下一代测序）进行测序，并使用各种生物信息学工具和软件来鉴定基因。在所有菌株中都鉴定到了乳杆菌素基因（plnA、plnD、plnF、plnI 和 plnJ）和调节对温度、pH、胆盐、渗透压和氧化应激反应的基因。乳杆菌可能是对抗抗菌药物耐药性的一种选择，并可能在未来取代有害的抗生素。
