Andrade P P, Schottelius J, Andrade C R
Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, Brasil.
Braz J Med Biol Res. 1988;21(3):517-21.
An enzyme-linked lectin assay (ELLA) based on the ELISA assay, using intact formalin-fixed promastigotes to coat poly-L-lysine-treated microtiter plates is described. The assay was used to study the lectin receptors of Leishmania donovani chagasi, L. donovani donovani and L. mexicana amazonensis. ConA, RCA, WGA, and PNA receptors were found in the three parasites. SBA receptors were found to be as frequent as the other receptors in L. donovani chagasi but not in the other two parasites which showed little SBA binding. Trypsin treatment of the two L. donovani subspecies did not remove any of the lectin receptors studied.
描述了一种基于酶联免疫吸附测定（ELISA）的酶联凝集素测定（ELLA），该方法使用完整的福尔马林固定前鞭毛体包被经聚-L-赖氨酸处理的微量滴定板。该测定用于研究杜氏利什曼原虫恰加斯亚种、杜氏利什曼原虫杜氏亚种和亚马逊利什曼原虫的凝集素受体。在这三种寄生虫中均发现了刀豆球蛋白A（ConA）、蓖麻凝集素（RCA）、小麦胚凝集素（WGA）和花生凝集素（PNA）受体。发现SBA受体在杜氏利什曼原虫恰加斯亚种中与其他受体一样常见，但在另外两种几乎没有SBA结合的寄生虫中则不然。对杜氏利什曼原虫的两个亚种进行胰蛋白酶处理并未去除所研究的任何凝集素受体。
