Saraiva E M, Andrade A F, Pereira M E
Eur J Cell Biol. 1986 Apr;40(2):219-25.
The cell surface carbohydrates of Leishmania mexicana amazonensis (amastigotes and promastigotes, both infective and non-infective forms) were comparatively analyzed by agglutination assay employing 28 highly purified lectins, and by binding assay using 125I-labeled lectins. Among the D-GalNAc binding lectins, Bandeiraea simplicifolia-I, Dolichos biflorus, Phaseolus vulgaris and Glycine max were highly specific for the amastigotes, while that from Maclura aurantiaca selectively agglutinated promastigotes. The lectins from Wistaria floribunda, Phaseolus lunatus (D-GalNAc), Arachis hypogaea (D-Gal) and Triticum vulgaris (D-GlcNAc) were selective for the infective forms (both amastigotes and promastigotes), not reacting with the non-infective ones. Conversely, no parasite agglutination occurred with the L-fucose binding lectins Lotus tetragonolobus and Ulex europaeus-I. Binding studies with 125I-labeled lectins from Wistaria floribunda, Triticum vulgaris and Arachis hypogaea were performed to find whether unagglutinated non-infective promastigotes might have receptors for these lectins, in which case absence of agglutination could be due to a peculiar arrangement of the receptors. These assays essentially confirmed the selectivity, demonstrated in the agglutination assays of these lectins for the infective promastigotes.
利用28种高度纯化的凝集素通过凝集试验，以及使用125I标记的凝集素通过结合试验，对墨西哥利什曼原虫亚马逊亚种（无鞭毛体和前鞭毛体，包括感染性和非感染性形式）的细胞表面碳水化合物进行了比较分析。在D-半乳糖胺结合凝集素中，简单豆科凝集素-I、双花扁豆、菜豆和大豆对无鞭毛体具有高度特异性，而桑橙凝集素则选择性地凝集前鞭毛体。紫藤、利马豆（D-半乳糖胺）、落花生（D-半乳糖）和普通小麦（D-葡萄糖胺）的凝集素对感染性形式（无鞭毛体和前鞭毛体）具有选择性，不与非感染性形式发生反应。相反，与L-岩藻糖结合凝集素四角百脉根和荆豆-I未发生寄生虫凝集现象。对来自紫藤、普通小麦和落花生的125I标记凝集素进行了结合研究，以确定未凝集的非感染性前鞭毛体是否可能具有这些凝集素的受体，在这种情况下，不发生凝集可能是由于受体的特殊排列。这些试验基本上证实了这些凝集素在凝集试验中对感染性前鞭毛体的选择性。
