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Polysaccharides immobilized in polyacrylamide gel as high-capacity adsorbents for Bandeiraea simplicifolia lectin and concanavalin A.

作者信息

Horisberger M

出版信息

Carbohydr Res. 1977 Feb;53(2):231-7. doi: 10.1016/s0008-6215(00)88091-x.

Abstract

A high-capacity adsorbent for lectins was easily prepared by immobilization of polysaccharides in a polyacrylamide gel. The properties of granulated guaran gel, used to purify by affinity chromatography the alpha-D-galactose-binding lectin from the seeds of Bandeiraea simplicifolia, were studied, as well as the binding of Concanavalin A (tetrameric) and succinylated Concanavalin A (dimeric) to a yeast mannan gel.

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