Department of Mechanical Engineering, University of California, Santa Barbara, CA 93106, USA.
Soft Matter. 2020 May 7;16(17):4192-4199. doi: 10.1039/c9sm02377a. Epub 2020 Apr 14.
We present a new approach to measuring cell-generated forces from the deformations of elastic microspheres embedded within multicellular aggregates. By directly fitting the measured sensor deformation to an analytical model based on experimental observations and invoking linear elasticity, we dramatically reduce the computational complexity of the problem, and directly obtain the full 3D mapping of surface stresses. Our approach imparts extraordinary computational efficiency, allowing tractions to be estimated within minutes and enabling rapid analysis of microsphere-based traction force microscopy data.
我们提出了一种新的方法来测量嵌入在多细胞聚集体中的弹性微球的变形来获得细胞产生的力。通过直接将测量得到的传感器变形拟合到一个基于实验观察和线性弹性的分析模型，我们极大地降低了问题的计算复杂度，并直接获得了表面应力的全 3D 映射。我们的方法赋予了非凡的计算效率，使得牵引力可以在几分钟内得到估计，并能够快速分析基于微球的牵引力显微镜数据。
