Santella R M
Cancer Center, School of Public Health, Columbia University, New York, New York 10032.
Cell Biol Toxicol. 1988 Dec;4(4):511-6. doi: 10.1007/BF00117779.
Sensitive immunologic techniques are now available for the detection and quantitation of carcinogen-DNA adducts. We have developed a number of specific monoclonal antibodies which recognize DNA modified by particular carcinogens, including benzo[a]pyrene, aminopyrene, 8-methoxypsoralen plus UVA light and vinyl chloride. These antibodies can be used in competitive enzyme-linked immunosorbent assays to quantitate adducts in DNA isolated from biological samples. Samples from treated animals as well as from humans with occupational or environmental exposure to carcinogens have been studied. In addition, antibodies can be used in indirect immunohistochemical studies to localize adduct formation in various tissues or cell types.
现在已有灵敏的免疫技术可用于检测和定量致癌物 - DNA加合物。我们已经开发出了多种特异性单克隆抗体，这些抗体能够识别由特定致癌物修饰的DNA，其中包括苯并[a]芘、氨基芘、8 - 甲氧基补骨脂素加紫外线A光以及氯乙烯。这些抗体可用于竞争性酶联免疫吸附测定，以定量从生物样品中分离出的DNA中的加合物。已经对来自经处理动物以及职业或环境接触致癌物的人类的样品进行了研究。此外，抗体可用于间接免疫组织化学研究，以定位各种组织或细胞类型中的加合物形成。
