Opt Lett. 2020 Apr 15;45(8):2247-2250. doi: 10.1364/OL.389300.
We report the development of a novel, to the best of our knowledge, fiber-based system to realize coregistered simultaneous acquisition of fluorescence lifetime (FL) data and Raman spectra from the same area. FL measurements by means of time-correlated single photon counting are realized with periodic out-of-phase external illumination of the field of view, enabling acquisition of data under bright illumination of the specimen. Raman measurements in the near-infrared are realized asynchronously. We present a detailed characterization of this technique and validate its potential to report intrinsic contrast. Fiber-based FL and Raman maps report complementary structural, compositional, and molecular contrast in biological tissues with diverse compositional features.
我们报告了一种新颖的基于光纤的系统的开发，据我们所知，该系统能够实现从同一区域同时采集荧光寿命（FL）数据和拉曼光谱的核心注册。通过对视场进行周期性的外相差照明，实现了基于时间相关单光子计数的 FL 测量，从而可以在标本的明场照明下采集数据。近红外的拉曼测量是异步实现的。我们对这项技术进行了详细的描述，并验证了其报告固有对比度的潜力。基于光纤的 FL 和拉曼图谱报告了具有不同组成特征的生物组织中具有互补结构、组成和分子对比度的图谱。
