DOE Center for Advanced Bioenergy and Bioproducts Innovation, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Sci Adv. 2022 Jul;8(26):eabo0902. doi: 10.1126/sciadv.abo0902. Epub 2022 Jun 29.
DNA aptamers have been widely used as biosensors for detecting a variety of targets. Despite decades of success, they have not been applied to monitor any targets in plants, even though plants are a major platform for providing oxygen, food, and sustainable products ranging from energy fuels to chemicals, and high-value products such as pharmaceuticals. A major barrier to progress is a lack of efficient methods to deliver DNA into plant cells. We herein report a thiol-mediated uptake method that more efficiently delivers DNA into and tobacco leaf cells than another state-of-the-art method, DNA nanostructures. Such a method allowed efficient delivery of a glucose DNA aptamer sensor into for sensing glucose. This demonstration opens a new avenue to apply DNA aptamer sensors for functional studies of various targets, including metabolites, plant hormones, metal ions, and proteins in plants for a better understanding of the biodistribution and regulation of these species and their functions.
DNA 适体已被广泛用作检测各种靶标的生物传感器。尽管取得了数十年的成功，但它们尚未应用于监测植物中的任何靶标，尽管植物是提供氧气、食物以及从能源燃料到化学品和高价值产品（如药品）等可持续产品的主要平台。进展的主要障碍是缺乏将 DNA 递送至植物细胞的有效方法。本文报告了一种巯基介导的摄取方法，该方法比另一种最先进的方法——DNA 纳米结构更有效地将 DNA 递送至拟南芥和烟草叶片细胞。该方法允许将葡萄糖 DNA 适体传感器有效地递送至拟南芥中以感测葡萄糖。这一演示为应用 DNA 适体传感器进行各种靶标（包括代谢物、植物激素、金属离子和植物中的蛋白质）的功能研究开辟了新途径，有助于更好地了解这些物质的生物分布和调控及其功能。
