为什么我们不能只用聚合酶链反应（PCR）呢？基因型检测与表型检测在抗菌药物耐药性检测中的作用。
Why Can't We Just Use PCR? The Role of Genotypic versus Phenotypic Testing for Antimicrobial Resistance Testing.
作者信息
Bard Jennifer Dien, Lee Francesca
机构信息
Department of Pathology and Laboratory Medicine, Children's Hospital Los Angeles, Los Angeles, California, USA.
Keck School of Medicine, University of Southern California, Los Angeles, California, USA.
出版信息
Clin Microbiol Newsl. 2018 Jun 1;40(11):87-95. doi: 10.1016/j.clinmicnews.2018.05.003. Epub 2018 May 22.
Abstract
There is a need for phenotypic susceptibility testing that is expeditious and that can be performed directly from clinical specimens. While rapid pathogen identification is important, it is the susceptibility result that is essential for antimicrobial optimization. The options for rapid susceptibility testing are limited, with the majority of commercial tests available offering genotypic resistance detection only. In this article, a laboratorian and a clinician discuss the benefits and limitations of genotypic and phenotypic susceptibility testing and provide examples of how results should be interpreted to maximize the clinical utility.
摘要
需要一种快速的、能够直接从临床标本进行的表型药敏试验。虽然快速病原体鉴定很重要，但药敏结果对于抗菌药物优化至关重要。快速药敏试验的选择有限，大多数现有的商业检测仅提供基因型耐药性检测。在本文中，一位检验师和一位临床医生讨论了基因型和表型药敏试验的益处和局限性，并举例说明了应如何解读结果以最大限度地提高临床实用性。
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