Faculty of Biomedical Engineering, Technion - Israel Institute of Technology, Haifa 3200003, Israel.
Faculty of Mechanical Engineering, Technion - Israel Institute of Technology, Haifa 3200003, Israel.
Proc Natl Acad Sci U S A. 2017 Jul 18;114(29):E5787-E5795. doi: 10.1073/pnas.1703736114. Epub 2017 Jun 26.
Antibiotic resistance is a major global health concern that requires action across all sectors of society. In particular, to allow conservative and effective use of antibiotics clinical settings require better diagnostic tools that provide rapid determination of antimicrobial susceptibility. We present a method for rapid and scalable antimicrobial susceptibility testing using stationary nanoliter droplet arrays that is capable of delivering results in approximately half the time of conventional methods, allowing its results to be used the same working day. In addition, we present an algorithm for automated data analysis and a multiplexing system promoting practicality and translatability for clinical settings. We test the efficacy of our approach on numerous clinical isolates and demonstrate a 2-d reduction in diagnostic time when testing bacteria isolated directly from urine samples.
抗生素耐药性是一个全球性的主要健康问题，需要社会各界采取行动。特别是，为了允许对抗生素进行保守和有效的使用，临床环境需要更好的诊断工具，以便快速确定抗菌药物敏感性。我们提出了一种使用固定纳米升液滴阵列进行快速和可扩展的抗菌药物敏感性测试的方法，该方法能够在大约一半的时间内提供常规方法的结果，从而使其结果可以在同一天使用。此外，我们还提出了一种用于自动化数据分析的算法和一个多路复用系统，以促进临床环境的实用性和可转移性。我们在许多临床分离株上测试了我们方法的效果，并在直接从尿液样本中分离的细菌测试中证明了诊断时间缩短了 2 天。
