Zou Rong, Zhang Feng, Chen Chunyan, Cai Changqun
Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan 411105, China.
College of Science, Hunan Agricultural University, Changsha, 410128, China.
Sens Actuators B Chem. 2019 Oct 1;296:126608. doi: 10.1016/j.snb.2019.05.085. Epub 2019 May 30.
A novel DNA-stabilized silver nanoclusters (AgNCs)-based label-free fluorescent platform for simultaneously detecting two human immunodeficiency virus oligonucleotides (HIV DNAs) was developed. The sensing platform was established based on fluorescence enhancement of guanine (G)-rich and the phenomenon in the process of two silver nanoclusters (AgNCs) forming a nanoclusters dimer. The probe (AgNCs/G) utilized for HIV-1 detection adopted an effective conformation based on enhancement effect of G-rich sequence (at 500 nm ex / 565 nm em) while the probe (AgNCs/AgNCs) for HIV-2 generated fluorescence signals (at 580 nm ex / 630 nm em) with bright fluorescence only in nanoclusters dimer. The nanoprobe shows high selectivity for multiplexed analysis of target DNA with a detection limit of 11 pM, respectively. Moreover, this is the first time to use the affectivity of fluorescent AgNCs and two HIV DNAs simultaneous detection integrated into a novel method, which shows a great promise in biomedical research and early clinical diagnosis.
开发了一种基于新型DNA稳定银纳米簇（AgNCs）的无标记荧光平台，用于同时检测两种人类免疫缺陷病毒寡核苷酸（HIV DNA）。该传感平台基于富含鸟嘌呤（G）的荧光增强以及两个银纳米簇（AgNCs）形成纳米簇二聚体过程中的现象而建立。用于HIV-1检测的探针（AgNCs/G）基于富含G序列的增强效应（在500nm激发/565nm发射）采用了有效的构象，而用于HIV-2的探针（AgNCs/AgNCs）仅在纳米簇二聚体中产生具有明亮荧光的荧光信号（在580nm激发/630nm发射）。该纳米探针在对目标DNA进行多重分析时显示出高选择性，检测限分别为11 pM。此外，这是首次将荧光AgNCs的有效性与两种HIV DNA的同时检测整合到一种新方法中，这在生物医学研究和早期临床诊断中显示出巨大的前景。
