Centre for Fish and Wildlife Health, 3012 Bern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Länggassstrasse 122, 3012 Bern, Switzerland.
Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, Switzerland.
Toxicol In Vitro. 2020 Aug;66:104859. doi: 10.1016/j.tiv.2020.104859. Epub 2020 Apr 11.
Emission of platinum nanoparticles (Pt NPs) especially from vehicle exhaust catalysts and pharmaceutics cause an increase in concentrations of this metal in aquatic environments. In this study, small (4-9 nm) uncoated and polyvinylpyrrolidone (PVP) coated Pt NPs were synthetized and their dispersion in different exposure media were evaluated. Pt NP uptake in two established fish cell lines were investigated and comparative in vitro cytotoxicity of Pt NPs and ions were assessed. The coated and uncoated Pt NPs dispersions in minimum essential medium (MEM) with fetal bovine serum (FBS) displayed high colloidal stability. Transmission electron microscopy (TEM) and high-resolution scanning electron microscope equipped with an energy-dispersive X-ray spectrometer (STEM/EDX) indicated no detectable cellular uptake of Pt NPs in both cell line monolayers. But with ICP-MS analysis, trace amount of Pt content was determined in all digested monolayer cell samples. The cytotoxicity of both Pt NPs and Pt ions on both fish cell lines after 48 h exposure was investigated through three assays to monitor different endpoints of cytotoxicity. In all studied concentrations (0.325-200 mg/L) no significant cytotoxicity (p > .5) compared to controls were observed in the cells exposed to coated Pt NPs. Uncoated Pt NP and ion exposed cells indicated similar concentration dependent cytotoxicity on both cell lines.
铂纳米粒子（Pt NPs）的排放，特别是来自车辆废气催化剂和药物的排放，导致这种金属在水生环境中的浓度增加。在这项研究中，我们合成了小尺寸（4-9nm）的未涂层和聚乙烯吡咯烷酮（PVP）涂层的 Pt NPs，并评估了它们在不同暴露介质中的分散情况。我们研究了两种已建立的鱼类细胞系中 Pt NP 的摄取，并评估了 Pt NPs 和离子的体外比较细胞毒性。在含有胎牛血清（FBS）的最低必需培养基（MEM）中，涂层和未涂层的 Pt NPs 分散体显示出高度的胶体稳定性。透射电子显微镜（TEM）和配备能量色散 X 射线光谱仪（STEM/EDX）的高分辨率扫描电子显微镜表明，在两种细胞系单层中均未检测到 Pt NPs 的可检测细胞摄取。但是，通过 ICP-MS 分析，在所有消化的单层细胞样品中均测定到痕量的 Pt 含量。通过三种检测方法研究了 48 小时暴露后 Pt NPs 和 Pt 离子对两种鱼类细胞系的细胞毒性，以监测细胞毒性的不同终点。在所有研究浓度（0.325-200mg/L）下，与对照组相比，暴露于涂层 Pt NPs 的细胞中未观察到明显的细胞毒性（p>.5）。未涂层 Pt NP 和离子暴露的细胞在两种细胞系上均显示出相似的浓度依赖性细胞毒性。
