水稻 OsBT1 通过糖代谢途径调控种子休眠。
Rice OsBT1 regulates seed dormancy through the glycometabolism pathway.
机构信息
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing, 210095, China.
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Research Center of Jiangsu Plant Gene Engineering, Nanjing Agricultural University, Nanjing, 210095, China; Department of Biology and Agricultural Engineering, Quynhon University, Quynhon, Binhdinh, 590000, Viet Nam.
出版信息
Plant Physiol Biochem. 2020 Jun;151:469-476. doi: 10.1016/j.plaphy.2020.03.055. Epub 2020 Apr 5.
Seed dormancy and germination in rice (Oryza sativa L.) are complex and important agronomic traits that involve a number of physiological processes and energy. A mutant named h470 selected from aCo-radiated indica cultivar N22 population had weakened dormancy that was insensitive to Gibberellin (GA) and Abscisic acid (ABA). The levels of GA and ABA were higher in h470 than in wild-type (WT) plants. The gene controlling seed dormancy in h470 was cloned by mut-map and transgenesis and confirmed to encode an ADP-glucose transporter protein. A 1 bp deletion in Os02g0202400 (OsBT1) caused the weaker seed dormancy in h470. Metabolomics analyses showed that most sugar components were higher in h470 seeds than the wild type. The mutation in h470 affected glycometabolism.
水稻（Oryza sativa L.）的种子休眠和萌发是复杂且重要的农艺性状，涉及许多生理过程和能量。从辐照籼稻品种 N22 群体中选择的突变体 h470 休眠减弱，对赤霉素（GA）和脱落酸（ABA）不敏感。h470 中的 GA 和 ABA 水平高于野生型（WT）植物。通过突变图谱和转基因克隆控制 h470 种子休眠的基因，并证实其编码 ADP-葡萄糖转运蛋白。Os02g0202400（OsBT1）中的 1 bp 缺失导致 h470 的种子休眠减弱。代谢组学分析表明，h470 种子中的大多数糖成分高于野生型。h470 的突变影响糖代谢。
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