Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, Anhui, China; Key Laboratory of Environmental Toxicology and Pollution Control Technology of Anhui Province, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230026, Anhui, China.
Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, Anhui, China; School of Life Sciences, University of Science and Technology of China, Hefei 230026, Anhui, China.
Bioresour Technol. 2020 Aug;309:123304. doi: 10.1016/j.biortech.2020.123304. Epub 2020 Apr 7.
In China, Dolichospermum flos-aquae is one of the most prevalent bloom-forming cyanobacteria and thus a major challenge for the concerned catchment area. To solve this problem and turn it into an opportunity for heavy metal remediation, we investigated the potential of D. flos-aquae for production algal biochar, and constructed a microbe-algal biochar composite. The microbe-biochar composite (biochar immobilized Proteus mirabilis PC801) showed superior hexavalent chromium removal capacity. It produced 100% Cr(VI) (150 mg/L) removal efficiency, with 87.7% total Cr immobilized in/on the particles and only 12.3% Cr(III) left in solution. Furthermore, Scanning electron microscopy-energy dispersive spectroscopy and antioxidase activity results showed that Cr(VI) reduction mainly occurred outside the cells, and the biochar can effectively protect P. mirabilis YC801 from the direct toxicity of chromium, thereby promoting the removal efficiency. Overall, this study provides a promising approach by utilizing this harmful algae for the bio-remediation of Cr(VI)-contaminated groundwater in practical application.
在中国，水华鱼腥藻是最常见的形成藻华的蓝藻之一，因此是相关集水区的主要挑战。为了解决这个问题，并将其转化为重金属修复的机会，我们研究了水华鱼腥藻生产藻类生物炭的潜力，并构建了微生物-藻类生物炭复合材料。微生物-生物炭复合材料（固定化奇异变形杆菌 PC801 的生物炭）表现出优异的六价铬去除能力。它对 150mg/L 的 Cr(VI) 产生了 100%的去除效率，其中 87.7%的总铬被固定在颗粒内/上，只有 12.3%的铬（III）留在溶液中。此外，扫描电子显微镜-能谱和抗氧化酶活性结果表明，Cr(VI)的还原主要发生在细胞外，生物炭可以有效地保护奇异变形杆菌 YC801 免受铬的直接毒性，从而提高去除效率。总的来说，这项研究为利用这种有害藻类在实际应用中生物修复六价铬污染地下水提供了一种有前景的方法。
