β-内酰胺类抗生素对[物种名称]小孢子胚胎发生的影响。

Effect of Beta-Lactam Antibiotics on Microspore Embryogenesis in Species.

作者信息

Mineykina Anna, Shumilina Daria, Bondareva Ludmila, Soldatenko Alexey, Domblides Elena

机构信息

Laboratory of Reproductive Biotechnology in Crop Breeding, Federal State Budgetary Scientific Institution Federal Scientific Vegetable Center (FSBSI), Selectsionnaya str., 14, Odintsovo district, 143072 Moscow region, Russia.

出版信息

Plants (Basel). 2020 Apr 10;9(4):489. doi: 10.3390/plants9040489.

DOI:10.3390/plants9040489

PMID:32290301

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7238966/

Abstract

Antibiotics are widely applied for plant cultivation in vitro to eliminate bacterial contamination. However, they can have both positive and negative effects on the cells of cultivated plants, and these effects largely depend on the type antibiotic used and its concentration. The objective of the present study was to estimate the effect of β-lactam antibiotics ampicillin (Amp) and cefotaxime (Cef) on microspore embryogenesis induction in vitro in the species. The performed experiments confirmed cefotaxime inhibits microspores in and , even in concentrations as low as 50 mg/L. The highest embryo yield was obtained for in the NLN-13 medium with added ampicillin in concentrations of 50-100 mg/L as an antimicrobial agent. This embryo yield was significantly higher than that obtained in a medium without supplemented antibiotics and two times higher than in the medium with added cefotaxime. Analogous results were obtained for and .

摘要

抗生素被广泛应用于植物离体培养中以消除细菌污染。然而，它们对培养植物的细胞可能既有正面影响也有负面影响，而且这些影响很大程度上取决于所用抗生素的类型及其浓度。本研究的目的是评估β-内酰胺类抗生素氨苄青霉素（Amp）和头孢噻肟（Cef）对该物种离体小孢子胚胎发生诱导的影响。所进行的实验证实，头孢噻肟即使在低至50 mg/L的浓度下也会抑制某植物和另一植物中的小孢子。在添加浓度为50 - 100 mg/L氨苄青霉素作为抗菌剂的NLN - 13培养基中，某植物获得了最高的胚胎产量。该胚胎产量显著高于未添加抗生素的培养基中的产量，且比添加头孢噻肟的培养基中的产量高出两倍。某植物和另一植物也获得了类似的结果。