Rotten D, Gavignet C, Colin M C, Robert A M, Godeau G
Departement de Gynécologie, Obstétrique et Biologie de la Reproduction, Faculté de Médecine, Université Paris XII-Val de Marne, Créteil, France.
Clin Physiol Biochem. 1988;6(5):285-92.
To assess the evolution of the elastic fiber network of the human uterine cervix before, during and after pregnancy, biopsy samples were obtained from 49 women. The high affinity of the polyphenolic compound (+)-catechin for elastin was used to stain the elastic fibers selectively, and enabled automated image analysis. In the human uterine cervix, the elastic fiber network is made up of: (1) fibers running parallel to the basement lamina of the epithelium, and (2) thinner, perpendicular fibers. Quantification using automated image analysis shows a decline in the cervical elastin content from a prepregnancy level of 1.33 +/- 0.08 (SEM) to 0.73 +/- 0.09% (Vv) at the end of pregnancy. In parallel with a constant decline, dissociation and disorganization of the fibers become more clearly evident as pregnancy progresses. However, by 5-7 weeks postpartum the elastic fiber network appears almost completely restructured. These changes support a role of elastin in the processes of cervical maturation and reconstruction during pregnancy and after delivery.
为评估人类子宫颈弹性纤维网络在妊娠前、妊娠期间和妊娠后的演变，从49名女性身上获取了活检样本。利用多酚化合物（+）-儿茶素对弹性蛋白的高亲和力来选择性地染色弹性纤维，并实现自动图像分析。在人类子宫颈中，弹性纤维网络由以下部分组成：（1）与上皮基底膜平行的纤维，以及（2）较细的垂直纤维。使用自动图像分析进行定量分析显示，宫颈弹性蛋白含量从妊娠前的1.33±0.08（SEM）下降至妊娠末期的0.73±0.09%（Vv）。随着妊娠进展，在弹性蛋白含量持续下降的同时，纤维的解离和紊乱变得更加明显。然而，产后5至7周时，弹性纤维网络几乎完全重新构建。这些变化支持了弹性蛋白在妊娠期间和分娩后子宫颈成熟和重建过程中的作用。
