Godeau G
Pathol Biol (Paris). 1984 Mar;32(3):215-6.
The staining methods proposed for the histological detection of elastic fibers are not entirely selective for the elastic fiber system and stain other tissue components also as for example nuclei and collagenous components. We worked out several staining methods which enable us to obtain a selective staining of all the elastic and pre-elastic fibers with no staining of the background by using in the staining solution polyphenols like: pyrogallol, (+) catechin, tannic acid and others. As the background is colourless, our method is suitable for the quantitation of elastic system fibers by automated computerized image analysis.
用于弹性纤维组织学检测的染色方法并非完全对弹性纤维系统具有选择性，也会对其他组织成分染色，比如细胞核和胶原成分。我们研发出了几种染色方法，通过在染色溶液中使用诸如连苯三酚、（+）儿茶素、单宁酸等多酚类物质，能够对所有弹性纤维和前弹性纤维进行选择性染色，且背景不着色。由于背景无色，我们的方法适用于通过自动化计算机图像分析对弹性系统纤维进行定量分析。
