Huang Jie, Ouyang Caiqun, Yang Senlin, Wei Wenting, Mao Limei
Department of Nutrition and Food Hygiene, School of Public Health, Southern Medical University, Guangzhou 510515, China.
Wei Sheng Yan Jiu. 2020 Jan;49(1):86-91. doi: 10.19813/j.cnki.weishengyanjiu.2020.01.015.
To investigate effects of different ratios of n-6/n-3 Polyunsaturated fatty acid(PUFA) on adiponectin, glycolipid metabolism and antioxidant capacity in high fat-diet fed rats.
Fixty male Wistar rats were randomly divided into control group(CON, 17% energy from fat, n-6/n-3 PUFA=5∶1) and high-fat treatment groups(1∶1 group∶n-6/n-3 PUFA=1∶1, 5∶1 group, n-6/n-3 PUFA=5∶1, 10∶1 group∶ n-6/n-3 PUFA=10∶1 and 20∶1 group∶ n-6/n-3 PUFA=20∶1, 42% energy from fat). Rats were fed for 12 week. Serum glucose and lipid(0, 4, 8 and 12 week) were detected. Antioxidant indexes(0 and 12 week), adiponectin mRNA and protein expressions in epididymal adipose tissues as well as serum adiponectin(12 week) were measured.
Baseline data showed no significant inter-group difference(P>0. 05). 1∶1 and 5∶1 showed no significant changes in serum glucose compared with control at all time points(P>0. 05), while 10∶1 and 20∶1 significantly increased it at the 12 th week(P<0. 05). At the 12 th week, total cholesterol(TC) and triglycerides(TG) in high-fat treatment groups were significantly higher than control(P<0. 01), with 20∶1 showing significant increment of TG compared with other high-fat treatment groups(P<0. 01). At the 12~(th) week, glutathione peroxidase and superoxide dismutase content in high-fat treatment groups all decreased significantly compared with control(P<0. 05), and 20∶1 decreased most. Malondialdehyde content significantly increased in high-fat treatment groups(P<0. 05), and 10∶1 increased most. Adiponectin mRNA expression significantly decreased in 20∶1 compared with control, 1∶1 and 5∶1(P<0. 05). Increment of adiponectin protein expression was significantly shown in 5∶1 compared with other high-fat treatment groups(P<0. 05), while it was most obviously decreased in 20∶1(P<0. 05).
Lower n-6/n-3 PUFA ratios(1∶1 and 5∶1) contributed to improvement of glycolipid metabolism and antioxidant capacity as well as increment of adiponectin expression of rats fed with high-fat diet.
探讨不同比例的n-6/n-3多不饱和脂肪酸（PUFA）对高脂饮食喂养大鼠脂联素、糖脂代谢及抗氧化能力的影响。
将60只雄性Wistar大鼠随机分为对照组（CON，脂肪供能17%，n-6/n-3 PUFA = 5∶1）和高脂处理组（1∶1组：n-6/n-3 PUFA = 1∶1；5∶1组：n-6/n-3 PUFA = 5∶1；10∶1组：n-6/n-3 PUFA = 10∶1；20∶1组：n-6/n-3 PUFA = 20∶1，脂肪供能42%）。大鼠喂养12周。检测0、4、8和12周时的血清葡萄糖和血脂。测定0和12周时的抗氧化指标、附睾脂肪组织中脂联素mRNA和蛋白表达以及12周时的血清脂联素。
基线数据显示组间无显著差异（P>0.05）。1∶1组和5∶1组在所有时间点的血清葡萄糖与对照组相比无显著变化（P>0.05），而10∶1组和20∶1组在第12周时显著升高（P<0.05）。在第12周时，高脂处理组的总胆固醇（TC）和甘油三酯（TG）显著高于对照组（P<0.01），20∶1组的TG与其他高脂处理组相比显著升高（P<0.01）。在第12周时，高脂处理组的谷胱甘肽过氧化物酶和超氧化物歧化酶含量与对照组相比均显著降低（P<0.05），20∶1组降低最多。高脂处理组的丙二醛含量显著增加（P<0.05），10∶1组增加最多。与对照组、1∶1组和5∶1组相比，2∶1组的脂联素mRNA表达显著降低（P<0.05）。与其他高脂处理组相比，5∶1组脂联素蛋白表达显著增加（P<0.05），而20∶1组降低最明显（P<0.05）。
较低的n-6/n-3 PUFA比例（1∶1和5∶1）有助于改善高脂饮食喂养大鼠的糖脂代谢和抗氧化能力，并增加脂联素表达。
