Yang Ligang, Song Zhixiu, Cao Weixin, Wang Yanyan, Lu Huixia, Guo Fei, Yang Huijun, Chen Jianshuang, Wang Shaokang, Sun Guiju
Wei Sheng Yan Jiu. 2016 May;45(3):436-41.
To investigate effects of diets with different fatty acid composition on serum lipid profiles, inflammation, oxidative stress and endothelial function in mice fed high-fat diets.
Male KM mice were randomly divided into five groups and were fed normal control diet, high-fat lard diet, high-fat diets with n-6/n-3 polyunsaturated fatty acid (PUFA) ratios of 1:1, 5:1 nd 2:1 for fiv weeks, respectively. The levels of serum triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C ) iterlekin-6 (IL-6), alonildehyde (MDA), hypersensitive C-reactive protein (hs-CRP), tumor ncrosis factor alpha (TNF-alpha), lipid lphaproxide (LPO), 8-iso postaglandin F2aalpha (8-iso-PGlphaF2u), oxidialphaed low-density lipoprotein (ox-LDL), free faty acid (FFA), E-selectin (ES) and von Willebrand factor (vWF) were measured.
The levels of serum LDL-C and non-HDL-C in the lard group were significantly higher than those in the other groups (P < 0.05). Th e ard group had. significntly higher serum TG and TC concentrations compared to 1:1 and 5: groups P <0. 05). The evels of serum FFA in 20:1 group wre significantly higher than those in 1:1 and 5:1 groups P < 0.05). Co mpred with the lard and 20:1 groups,the 1:1 and 5:1 groups displayed lower levels of inflammatory cytokines, oxidative stress and ES. The 5:1 group sgnificantly decreased the level of serum vWF compared to lard and 20:1 groups P < 0.05).
diet with low n-6/n-3 PUFA ratio could improve lipid metabolism, inflammation, oxidative stress and endothelial function compared to high-fat diets with lard and higher n-6/n-3 PUFA ratio. The diet with low n-6/n-3 PUFA ratio can improve cardiovascular disease risk factors to prevent cardiovascular disease.
研究不同脂肪酸组成的饮食对高脂饮食喂养小鼠血清脂质谱、炎症、氧化应激和内皮功能的影响。
将雄性KM小鼠随机分为五组，分别给予正常对照饮食、高脂猪油饮食、n-6/n-3多不饱和脂肪酸（PUFA）比例为1:1、5:1和2:1的高脂饮食，持续五周。检测血清甘油三酯（TG）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、白细胞介素-6（IL-6）、丙二醛（MDA）、超敏C反应蛋白（hs-CRP）、肿瘤坏死因子α（TNF-α）、脂质过氧化物（LPO）、8-异前列腺素F2α（8-iso-PGF2α）、氧化型低密度脂蛋白（ox-LDL）、游离脂肪酸（FFA）、E-选择素（ES）和血管性血友病因子（vWF）水平。
猪油组血清LDL-C和非HDL-C水平显著高于其他组（P<0.05）。与1:1和5:1组相比，猪油组血清TG和TC浓度显著更高（P<0.05）。2:1组血清FFA水平显著高于1:1和5:1组（P<0.05）。与猪油组和2:1组相比，1:1和5:1组炎症细胞因子、氧化应激和ES水平较低。与猪油组和2:1组相比，5:1组血清vWF水平显著降低（P<0.05）。
与含猪油且n-6/n-3 PUFA比例较高的高脂饮食相比，n-6/n-3 PUFA比例低的饮食可改善脂质代谢、炎症、氧化应激和内皮功能。n-6/n-3 PUFA比例低的饮食可改善心血管疾病危险因素以预防心血管疾病。
