Boone Erin C, Xiao Hua, Vierling Michael M, Decker Logan M, Sy Victor T, Kennedy Rana F, Bonham Marilyn A, Schmitz Shannon F, John Annie M, Hammond Thomas M, Shiu Patrick K T
Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211 and.
School of Biological Sciences, Illinois State University, Normal, Illinois 61790.
G3 (Bethesda). 2020 Jun 1;10(6):1919-1927. doi: 10.1534/g3.120.401236.
In the filamentous fungus , genes unpaired during meiosis are silenced by a process known as meiotic silencing by unpaired DNA (MSUD). MSUD utilizes common RNA interference (RNAi) proteins, such as Dicer and Argonaute, to target homologous mRNAs for silencing. Previously, we demonstrated that nuclear cap-binding proteins NCBP1 and NCBP2 are involved in MSUD. We report here that SAD-8, a protein similar to human NCBP3, also mediates silencing. Although SAD-8 is not essential for either vegetative or sexual development, it is required for MSUD. SAD-8 localizes predominantly in the nucleus and interacts with both NCBP1 and NCBP2. Similar to NCBP1 and NCBP2, SAD-8 interacts with a component (Argonaute) of the perinuclear meiotic silencing complex (MSC), further implicating the involvement of cap-binding proteins in silencing.
在丝状真菌中，减数分裂期间未配对的基因会通过一种称为“未配对DNA介导的减数分裂沉默”（MSUD）的过程被沉默。MSUD利用常见的RNA干扰（RNAi）蛋白，如Dicer和Argonaute，来靶向同源mRNA进行沉默。此前，我们证明了核帽结合蛋白NCBP1和NCBP2参与MSUD。我们在此报告，与人类NCBP3相似的蛋白质SAD-8也介导沉默。尽管SAD-8对于营养生长或有性发育都不是必需的，但MSUD需要它。SAD-8主要定位于细胞核，并与NCBP1和NCBP2相互作用。与NCBP1和NCBP2类似，SAD-8与核周减数分裂沉默复合体（MSC）的一个组分（Argonaute）相互作用，进一步表明帽结合蛋白参与沉默过程。
