Decker Logan M, Boone Erin C, Xiao Hua, Shanker Benjamin S, Boone Shannon F, Kingston Shanika L, Lee Seung A, Hammond Thomas M, Shiu Patrick K T
Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211.
Department of Biology, Barry University, Miami Shores, Florida 33161.
Genetics. 2015 Apr;199(4):1017-21. doi: 10.1534/genetics.115.174623. Epub 2015 Feb 2.
In Neurospora, genes not paired during meiosis are targeted by meiotic silencing by unpaired DNA (MSUD). Here, our bimolecular fluorescence complementation (BiFC) study suggests that RNA-directed RNA polymerase, Dicer, Argonaute, and others form a silencing complex in the perinuclear region, with intimate interactions among the majority of them. We have also shown that SAD-2 is likely the anchor for this assembly.
在粗糙脉孢菌中，减数分裂期间未配对的基因会被未配对DNA介导的减数分裂沉默（MSUD）靶向。在此，我们的双分子荧光互补（BiFC）研究表明，RNA依赖性RNA聚合酶、Dicer、Argonaute等在核周区域形成一个沉默复合体，其中大多数成员之间存在密切相互作用。我们还表明，SAD-2可能是该复合体组装的锚定蛋白。
