Grupo de Espectroscopia Vibracional y Materiales Moleculares, Instituto de Química, Pontificia Universidad Católica De Valparaíso, Valparaíso, Chile.
Laboratorio de Patología de Organismos Acuáticos y Biotecnología Acuicola, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Viña del Mar, Chile.
J Fish Dis. 2020 May;43(5):621-629. doi: 10.1111/jfd.13159. Epub 2020 Apr 15.
Vibriosis outbreaks due to Vibrio ordalii occur globally, but Chilean salmon aquaculture, in particular, has suffered significant monetary losses in the last 15 years. Little is known about the virulence mechanisms employed by V. ordalii. However, most Vibrio pathogens (e.g., Vibrio anguillarum, a very close taxonomic species) present outer membrane vesicles (OMVs) that are released extracellularly and implicated in the delivery of virulence factors to host cells. This study provides the first reported evidence of the fish pathogen V. ordalii producing and releasing OMVs under normal growth conditions. Analyses were conducted with the V. ordalii strain Vo-LM-18 and the type strain ATCC 33509 . For comparative purposes, the reference strain V. anguillarum ATCC 43307 was employed. The average size for the three Vibrio strains was 0.215 ± 0.6 µm (via scanning electron microscopy) or between 0.19 and 1.8 µm (via dynamic light scattering), with each bacterium presenting a wide range. SDS-PAGE revealed similarities in OMV patterns, but neither total nor external proteins were identical. Comparing V. ordalii ATCC 33509 and Vo-LM-18, bands were most evident in the total proteins, and the greatest degree of similarity in OMV profiles was between 37 and 50 kDa. The purified OMVs demonstrated haemolytic enzyme activity, which could play a role during V. ordalii infection. These data represent an initial step towards gaining new insights into this virulence factor, of which a lot is known in other pathogenic microorganisms.
由弧菌属弧菌引起的弧菌病在全球范围内爆发，但智利的鲑鱼养殖业在过去 15 年中遭受了重大的经济损失。目前人们对弧菌属弧菌所采用的毒力机制知之甚少。然而，大多数弧菌病原体（例如，非常接近的分类物种鳗弧菌）存在外膜囊泡（OMV），这些囊泡被释放到细胞外，并被认为在将毒力因子递送到宿主细胞中发挥作用。本研究首次报道了鱼类病原体弧菌属弧菌在正常生长条件下产生和释放 OMV 的证据。使用弧菌属弧菌菌株 Vo-LM-18 和模式菌株 ATCC 33509 进行了分析。为了进行比较，还使用了参考菌株鳗弧菌 ATCC 43307。三种弧菌菌株的平均大小为 0.215±0.6µm（通过扫描电子显微镜）或 0.19 至 1.8µm（通过动态光散射），每个细菌的大小范围都很宽。SDS-PAGE 显示 OMV 图谱相似，但总蛋白和外膜蛋白都不相同。比较弧菌属弧菌 ATCC 33509 和 Vo-LM-18，总蛋白中存在最明显的条带，并且 OMV 图谱之间的相似性最大，在 37 至 50 kDa 之间。纯化的 OMV 显示出溶血酶活性，这在弧菌属弧菌感染期间可能发挥作用。这些数据代表了朝着深入了解这种毒力因子迈出的第一步，而其他致病性微生物中对此已有很多了解。
