Department of Pharmaceutics, ISF College of Pharmacy, Moga, Punjab, India.
Department of Science and Technology, Maharaja Ranjit Singh Punjab Technical University, Bathinda, Punjab, India.
J Microencapsul. 2020 Aug;37(5):341-354. doi: 10.1080/02652048.2020.1756970. Epub 2020 Apr 23.
The aim of this project is to improve the therapeutic effectiveness, permeation and retention of clobetasol propionate in sebaceous glands by reporting the use of Squarticles as lipidic nanosystem. Homogenisation method is used for the formulation of Squarticles (nanoemulgel) which was characterised on the basis of size, polydispersity index (PDI), viscosity, spreadability, DSC, % release, skin permeation deposition studies, and studies, scanning electron microscopic (SEM) and physical storage stability studies were done at different temperature conditions, i.e. 4 ± 2 °C, 25 ± 2 °C and 45 ± 2 °C for a period of 6 months for drug and formulation. The morphological characterisation of prepared nanoemulsion shows small spherical shape and uniform size distribution as observed in the Scanning electron microscopic (SEM), having mean size (240.5 ± 9.2) and mean size distribution (0.282 ± 0.03) and zeta potential (-51.21). The drug release from optimised nanoemulsion (F2) in PBS (pH 5.5) was approximately 84.24 ± 1.35%, nanoemulgel formulations showed the release of 66.83 ± 2.05% while marketed gel showed the release of 57.67 ± 1.63% after 24 h. The cumulative percentage retention of clobetasol propionate loaded nanoemulgel was 63 ± 1.28% which was more than the marketed formulation (23.12% ±0.54). Physical stability studies show that formulation is more stable in cold condition. Further, the stability of active ingredient in gel formulation was determined using HPLC which shows around 15 ± 0.84% of loss in its activity. The present work has demonstrated the use of Squarticles as a novel carrier for treatment of plaque psoriasis by enhancing the better permeation, increasing skin retention, and enhances the effect of drug. The study also shows that the formulation is more stable in cold condition.
本项目旨在通过报告使用 Squarticles（类脂纳米系统）来提高丙酸氯倍他索在皮脂腺中的治疗效果、渗透和保留。采用均质法制备 Squarticles（纳米乳凝胶），并基于粒径、多分散指数（PDI）、粘度、铺展性、差示扫描量热法（DSC）、%释放度、皮肤渗透沉积研究、扫描电子显微镜（SEM）和物理储存稳定性研究对其进行了表征，在不同温度条件下（即 4±2°C、25±2°C 和 45±2°C）进行了为期 6 个月的药物和制剂研究。所制备的纳米乳的形态学特征表明，在扫描电子显微镜（SEM）中观察到的小的球形形状和均匀的粒径分布，具有平均粒径（240.5±9.2）和平均粒径分布（0.282±0.03）和 Zeta 电位（-51.21）。在 PBS（pH 5.5）中从优化的纳米乳（F2）中释放药物约 84.24±1.35%，纳米乳凝胶制剂显示 66.83±2.05%的释放，而市售凝胶在 24 小时后显示 57.67±1.63%的释放。负载丙酸氯倍他索的纳米乳凝胶的累积保留率为 63±1.28%，高于市售制剂（23.12%±0.54%）。物理稳定性研究表明，制剂在低温条件下更稳定。此外，还使用 HPLC 测定了凝胶制剂中活性成分的稳定性，结果表明其活性损失约为 15±0.84%。本工作证明了 Squarticles 作为一种新型载体用于治疗斑块状银屑病的用途，通过增强更好的渗透、增加皮肤保留和增强药物的效果。该研究还表明，制剂在低温条件下更稳定。
