Dust, Ventilation, and Toxic Substances Branch, Pittsburgh Mining Research Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Pittsburgh, Pennsylvania.
Chemical and Biological Monitoring Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention, Cincinnati, Ohio.
J Occup Environ Hyg. 2020 Jun;17(6):301-311. doi: 10.1080/15459624.2020.1742915. Epub 2020 Apr 15.
The objective of this study was to investigate the efficacy of an aerodynamic separation scheme for obtaining aerosols with nearly monodisperse fiber lengths as test samples for mechanistic toxicological evaluations. The approach involved the separation of aerosolized glass fibers using an Aerodynamic Aerosol Classifier (AAC) or a multi-cyclone sampling array, followed by the collection of separated samples on filter substrates, and the measurement of each sample fiber length distribution. A glass fiber aerosol with a narrow range of aerodynamic sizes was selected and sampled with the AAC or multi-cyclone sampling array in two separate setups. The fiber length and diameter were measured using a field emission scanning electron microscope. The glass fiber aerosol was separated in distinct groups of eight with the AAC and of four with the multi-cyclone sampling array. The geometric standard deviations of the fiber length distributions of the separated aerosols ranged from 1.49 to 1.69 for the AAC and from 1.6 to 1.8 for multi-cyclone sampling array. While the separation of glass fiber aerosols with an AAC is likely to produce two different length fiber groups and the length resolution may be acceptable, the overall mass throughput of these separation schemes is limited.
本研究旨在探讨气动分离方案在获得具有近乎单分散纤维长度的气溶胶方面的效果，这些气溶胶可作为机制毒理学评估的测试样本。该方法包括使用气动气溶胶分级器（AAC）或多旋风采样器对气溶胶化的玻璃纤维进行分离，然后将分离的样品收集在过滤基底上，并测量每个样品的纤维长度分布。选择具有较窄气动尺寸范围的玻璃纤维气溶胶，并使用 AAC 或多旋风采样器在两个独立的设置中进行采样。使用场发射扫描电子显微镜测量纤维长度和直径。玻璃纤维气溶胶在 AAC 中分为八组，在多旋风采样器中分为四组。分离气溶胶的纤维长度分布的几何标准偏差对于 AAC 为 1.49 至 1.69，对于多旋风采样器为 1.6 至 1.8。虽然使用 AAC 分离玻璃纤维气溶胶可能会产生两个不同长度的纤维组，并且长度分辨率可能是可接受的，但这些分离方案的总体质量通量是有限的。
