Fife Austin N, Cruzado Karin, Rashed Arash, Novy Richard G, Wenninger Erik J
Kimberly Research and Extension Center, University of Idaho, Kimberly, ID.
Aberdeen Research and Extension Center, University of Idaho, Aberdeen, ID.
J Insect Sci. 2020 Mar 1;20(2). doi: 10.1093/jisesa/ieaa020.
The potato/tomato psyllid Bactericera cockerelli (Šulc) transmits 'Candidatus Liberibacter solanacearum' (Lso) (also known as 'Candidatus Liberibacter psyllaurous'), the bacterium associated with zebra chip disease (ZC) in potato. When disease incidence is high, ZC causes large economic losses through reductions in potato yield and tuber quality. No commercial potato variety has been found totally resistant to the pathogen. We evaluated host acceptance behaviors using no-choice assays on three breeding clones derived from Solanum chacoense Bitter with putative tolerance to Lso and/or ZC as part of an effort to determine whether the disease tolerance observed in those breeding clones was related to effects on psyllid settling behavior. We also counted the number of eggs laid and nymphs hatched on the different genotypes to observe any differences in reproduction. The potato variety 'Russet Burbank' was used as a susceptible control. Probing frequency and female walking duration were greater on Russet Burbank than the other genotypes. Oviposition did not differ among genotypes. However, female psyllids on two of the Lso-tolerant genotypes displayed reduced fertility 18-24 d after confinement with a male, relative to females on Russet Burbank. These results suggest that although the germplasms display minor abiotic activity on psyllid fertility, tolerance to Lso may be more strongly linked with plant tolerance to the pathogen rather than effects on host acceptance behaviors.
马铃薯/番茄木虱Bactericera cockerelli（舒尔克）传播“Ca. Liberibacter solanacearum”（Lso）（也称为“Ca. Liberibacter psyllaurous”），这种细菌与马铃薯的斑马薯片病（ZC）有关。当病害发生率很高时，ZC会通过降低马铃薯产量和块茎质量造成巨大经济损失。尚未发现对该病原体完全抗性的商业马铃薯品种。我们使用无选择试验评估了来自苦味茄（Solanum chacoense Bitter）的三个具有假定Lso和/或ZC耐受性的育种克隆的寄主接受行为，作为确定在这些育种克隆中观察到的病害耐受性是否与对木虱定居行为的影响相关的努力的一部分。我们还统计了在不同基因型上产下的卵数和孵化的若虫数，以观察繁殖方面的任何差异。马铃薯品种“褐皮伯班克”用作感病对照。与其他基因型相比，褐皮伯班克上的刺探频率和雌虫行走持续时间更高。不同基因型之间的产卵情况没有差异。然而，相对于褐皮伯班克上的雌虫，在两种耐Lso基因型上的雌虫在与雄虫禁闭18 - 24天后显示出繁殖力降低。这些结果表明，虽然种质对木虱繁殖力表现出轻微的非生物活性，但对Lso的耐受性可能与植物对病原体的耐受性更密切相关，而不是对寄主接受行为的影响。
