Department of Chemistry and Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
Cell Chem Biol. 2020 Apr 16;27(4):365-375. doi: 10.1016/j.chembiol.2020.03.013.
Ferroptosis is a recently described form of cell death driven by iron-dependent lipid peroxidation. This type of cell death was first observed in response to treatment of tumor cells with a small-molecule chemical probe named erastin. Most subsequent advances in understanding the mechanisms governing ferroptosis involved the use of genetic screens and small-molecule probes. We describe herein the utility and limitations of chemical probes that have been used to analyze and perturb ferroptosis, as well as mechanistic studies of ferroptosis that benefitted from the use of these probes and genetic screens. We also suggest probes for ferroptosis and highlight mechanistic questions surrounding this form of cell death that will be a high priority for exploration in the future.
铁死亡是一种新近描述的细胞死亡形式，由铁依赖性脂质过氧化驱动。这种细胞死亡类型最初是在肿瘤细胞用小分子化学探针 erastin 处理时观察到的。随后在理解调控铁死亡的机制方面的大多数进展都涉及到遗传筛选和小分子探针的使用。本文描述了用于分析和扰乱铁死亡的化学探针的用途和局限性，以及受益于这些探针和遗传筛选的铁死亡的机制研究。我们还为铁死亡提出了探针，并强调了围绕这种细胞死亡形式的机制问题，这些问题将是未来探索的重点。
