用红木提取物和蓝色发光二极管进行抗菌光动力疗法减轻口臭——一项随机对照临床试验

Antimicrobial photodynamic therapy with Bixa orellana extract and blue LED in the reduction of halitosis-A randomized, controlled clinical trial.

作者信息

Gonçalves Marcela Leticia Leal, da Mota Ana Carolina Costa, Deana Alessandro Melo, Cavalcante Lisyanne Araújo de Souza, Horliana Anna Carolina Ratto Tempestini, Pavani Christiane, Motta Lara Jansiski, Fernandes Kristianne Porta Santos, Mesquita-Ferrari Raquel Agnelli, da Silva Daniela Fátima Teixeira, Motta Pamella de Barros, Araújo Prates Renato, Bussadori Sandra Kalil

机构信息

Postgraduate Program in Biophotonics Applied to Health Sciences, Nove de Julho University, Vergueiro Street, 235/249 - Liberdade, ZIP 01504-001, São Paulo, SP, Brazil.

出版信息

Photodiagnosis Photodyn Ther. 2020 Jun;30:101751. doi: 10.1016/j.pdpdt.2020.101751. Epub 2020 Apr 12.

DOI:10.1016/j.pdpdt.2020.101751

PMID:32294559

Abstract

BACKGROUND

This study aimed to evaluate the reduction of halitosis when using antimicrobial photodynamic therapy (aPDT) with Bixa orellana extract and blue light-emitting diode (LED).

METHODS

Forty-four UNINOVE students or employees with a diagnosis of sulfide (H2S) ≥ 112 ppb in gas chromatography were selected. The patients were randomly divided in groups: Group 1 (n = 15): aPDT with annatto and LED; Group 2 (n = 14): tongue scraping; Group 3 (n = 15): tongue scraping and aPDT. For aPDT, a wasBixa orellana extract used in a concentration of 20 % w/v (Fórmula e Ação®, São Paulo, Brazil) on the tongue for 2 min, associated with a blue-violet LED (Valo Cordless Ultradent® Products, Inc., South Jordan, UT, USA) (395-480 nm). Six points were irradiated on the back of the tongue, at wavelength 395-480 nm for 20 s, energy of 9.6 J and radiant energy of 6.37 J/cm per point. The results were compared before, immediately after treatment and 7 days after. The Friedman test was used for the intragroup analysis and the Kruskal Wallis test for the intergroup analysis.

RESULTS

In all groups, there was a difference between baseline and the value immediately after the treatment. In Groups 1 and 3, there was no difference between the baseline and the 7 days control.

CONCLUSION

There was an immediate reduction of halitosis, but the reduction was not maintained after 7days.

摘要

背景

本研究旨在评估使用含红木提取物的抗菌光动力疗法（aPDT）和蓝光发光二极管（LED）时口臭的减轻情况。

方法

选取44名经气相色谱诊断为硫化物（H2S）≥112 ppb的UNINOVE学生或员工。将患者随机分为几组：第1组（n = 15）：使用红木和LED进行aPDT；第2组（n = 14）：舌面清洁；第3组（n = 15）：舌面清洁和aPDT。对于aPDT，将浓度为20% w/v的红木提取物（Fórmula e Ação®，巴西圣保罗）涂于舌面2分钟，并与蓝紫色LED（Valo Cordless Ultradent® Products, Inc.，美国犹他州南乔丹）（395 - 480 nm）联合使用。在舌背照射6个点，波长为395 - 480 nm，照射20秒，能量为9.6 J，每个点的辐射能量为6.37 J/cm。在治疗前、治疗后即刻和治疗后7天比较结果。组内分析采用Friedman检验，组间分析采用Kruskal Wallis检验。