He Libang, Bi Yi, Wang Ruolan, Pan Chuanying, Chen Hong, Lan Xianyong, Qu Lei
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China.
Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, Shaanxi, China.
Animals (Basel). 2020 Apr 13;10(4):672. doi: 10.3390/ani10040672.
The SRY-type HMG box 9 () gene plays an important role in chondrocyte development as well as changes in hypertrophic chondrocytes, indicating that can regulate growth in animals. However, no studies to date have examined the correlation between variations in and growth traits in goats. Here, we found a 4 bp indel in the 3'UTR of and verified its association with growth traits in Shaanbei white cashmere goats ( = 1109). The frequencies of two genotypes (ID and II) were 0.397 and 0.603, respectively, and polymorphic information content (PIC) values showed that the indel had a medium PIC (PIC > 0.25). The 4 bp indel was significantly correlated with body length ( = 0.006), heart girth ( = 0.001), and hip width ( = 4.37 × 10 ). Notably, individuals with the ID genotype had significantly superior phenotypic traits compared with individuals bearing the II genotype. Hence, we speculated that the 4 bp indel is an important mutation affecting growth traits in goat, and may serve as an effective DNA molecular marker for marker-assisted selection in goat breeding programs.
SRY 型 HMG 盒 9（）基因在软骨细胞发育以及肥大软骨细胞变化中发挥着重要作用，表明其可调控动物生长。然而，迄今为止尚无研究探讨该基因变异与山羊生长性状之间的相关性。在此，我们在该基因的 3'UTR 区域发现了一个 4 bp 的插入/缺失，并在陕北白绒山羊（n = 1109）中验证了其与生长性状的关联。两种基因型（ID 和 II）的频率分别为 0.397 和 0.603，多态信息含量（PIC）值表明该插入/缺失具有中等 PIC（PIC > 0.25）。该 4 bp 的插入/缺失与体长（P = 0.006）、胸围（P = 0.001）和臀宽（P = 4.37×10）显著相关。值得注意的是，与 II 基因型个体相比，ID 基因型个体具有显著更优的表型性状。因此，我们推测该 4 bp 的插入/缺失是影响山羊生长性状的一个重要突变，并且可能作为山羊育种计划中标记辅助选择的有效 DNA 分子标记。
