利用沸石前体获得的磁性纳米复合材料从人血中分离生物实体。
Separation of Biological Entities From Human Blood by Using Magnetic Nanocomposites Obtained From Zeolite Precursors.
机构信息
Department of Applied Science and Technology and INSTM Unit of Torino-Politecnico, Corso Duca degli Abruzzi 24, Politecnico di Torino, 10129 Torino, Italy.
Department of Civil and Mechanical Engineering and INSTM Research Unit, Università degli Studi di Cassino e del Lazio Meridionale, Via G. Di Biasio 43, 03043 Cassino, FR, Italy.
出版信息
Molecules. 2020 Apr 14;25(8):1803. doi: 10.3390/molecules25081803.
In this work, three novel magnetic metal-ceramic nanocomposites were obtained by thermally treating Fe-exchanged zeolites (either A or X) under reducing atmosphere at relatively mild temperatures (750-800 °C). The so-obtained materials were thoroughly characterized from the point of view of their physico-chemical properties and, then, used as magnetic adsorbents in the separation of the target gene factors V and RNASE and of the bacteria DNA from human blood. Such results were compared with those obtained by using a top ranking commercial separation system (namely, SiMAG-N-DNA by Chemicell). The results obtained by using the novel magnetic adsorbents were similar to (or even better than) those obtained by using the commercial system, both during manual and automated separations, provided that a proper protocol was adopted. Particularly, the novel magnetic adsorbents showed high sensitivity during tests performed with small volumes of blood. Finally, the feasible production of such magnetic adsorbents by an industrial process was envisaged as well.
在这项工作中，通过在相对温和的温度（750-800°C）下在还原气氛中热处理交换了铁的沸石（A 或 X），获得了三种新型磁性金属-陶瓷纳米复合材料。从物理化学性质的角度对所获得的材料进行了彻底的表征，然后将其用作磁性吸附剂，用于从人血中分离靶基因因子 V 和 RNASE 以及细菌 DNA。将这些结果与使用顶级商业分离系统（即 Chemicell 的 SiMAG-N-DNA）获得的结果进行了比较。使用新型磁性吸附剂获得的结果与使用商业系统获得的结果相似（甚至更好），无论是手动分离还是自动分离，只要采用适当的方案即可。特别地，新型磁性吸附剂在使用小体积血液进行的测试中显示出很高的灵敏度。最后，还设想了通过工业过程可行地生产这种磁性吸附剂。
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