Department of Critical Care Medicine, Renmin Hospital and Wuhan University, Wu Han, 430071, China.
Pulmonary and Critical Care Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, 02115, USA.
Sci Rep. 2020 Apr 15;10(1):6480. doi: 10.1038/s41598-020-63225-y.
Precision-cut lung slices (PCLS) are ideal for measuring small airway contraction. However, these measurements are currently limited to acute exposure scenarios that typically last a few minutes to a few hours. Using an insulin-supplemented culture medium, we prolong the small airway contractility in mouse PCLS for up to two weeks. Compared to conventional culture medium, insulin-supplemented culture medium provides no additional benefit in preserving cellular viability or airway structure. However, it protects the airway smooth muscle (ASM) against a loss of smooth muscle myosin heavy chain (SMMHC) expression. We elucidate the significance of this new culture medium for chronic disease modeling of IL-13-induced airway hyper-responsiveness.
精密切割肺切片 (PCLS) 是测量小气道收缩的理想选择。然而，这些测量目前仅限于急性暴露场景，通常持续几分钟到几个小时。我们使用添加胰岛素的培养基将小鼠 PCLS 中的小气道收缩性延长至长达两周。与传统培养基相比，添加胰岛素的培养基在保持细胞活力或气道结构方面没有额外的益处。然而，它可以防止气道平滑肌 (ASM) 丢失平滑肌肌球蛋白重链 (SMMHC) 的表达。我们阐明了这种新培养基在 IL-13 诱导的气道高反应性慢性疾病建模中的意义。
