Igarashi Miki, Morimoto Miku, Suto Asuka, Nakatani Akiho, Hayakawa Tetsuhiko, Hara Kenjirou, Kimura Ikuo
Department of Applied Biological Science, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo, Japan.
Fuji Nihon Seito Corporation, Shizuoka, Japan.
PeerJ. 2020 Apr 8;8:e8893. doi: 10.7717/peerj.8893. eCollection 2020.
Dietary fiber, including inulin, promotes health via fermentation products, such as short-chain fatty acids (SCFAs), produced from the fiber by gut microbiota. SCFAs exert positive physiological effects on energy metabolism, gut immunity, and the nervous system. Most of the commercial inulin is extracted from plant sources such as chicory roots, but it can also be enzymatically synthesized from sucrose using inulin producing enzymes. Studies conducted on rodents fed with a cafeteria diet have suggested that while increasing plasma propionic acid, synthetic inulin modulates glucose and lipid metabolism in the same manner as natural inulin. Therefore, this study aimed to determine the effects of a synthetic inulin, Fuji FF, on energy metabolism, fecal SCFA production, and microbiota profiles in mice fed with a high-fat/high-sucrose diet.
Three-week-old male C57BL/6J mice were fed a high-fat/high-sucrose diet containing cellulose or Fuji FF for 12 weeks, and the effects on energy metabolism, SCFA production, and microbiota profiles were evaluated.
Body weight gain was inhibited by Fuji FF supplementation in high-fat/high-sucrose diet-fed C57BL/6J mice by reducing white adipose tissue weight while increasing energy expenditure, compared with the mice supplemented with cellulose. Fuji FF also elevated levels of acetic, propionic and butyric acids in mouse feces and increased plasma propionic acid levels in mice. Moreover, 16S rRNA gene amplicon sequencing of fecal samples revealed an elevated abundance of Bacteroidetes and a reduced abundance of Firmicutes at the phylum level in mice supplemented with Fuji FF compared to those supplemented with cellulose. Fuji FF also resulted in abundance of the family Bacteroidales S24-7 and reduction of Desulfovibrionaceae in the feces.
Long term consumption of Fuji FF improved the gut environment in mice by altering the composition of the microbiota and increasing SCFA production, which might be associated with its anti-obesity effects.
膳食纤维，包括菊粉，通过肠道微生物群从纤维产生的发酵产物，如短链脂肪酸（SCFAs）来促进健康。短链脂肪酸对能量代谢、肠道免疫和神经系统具有积极的生理作用。大多数商业菊粉是从植物来源如菊苣根中提取的，但也可以使用产菊粉酶从蔗糖中酶促合成。对喂食自助餐饮食的啮齿动物进行的研究表明，虽然合成菊粉会增加血浆丙酸水平，但它调节葡萄糖和脂质代谢的方式与天然菊粉相同。因此，本研究旨在确定合成菊粉富士FF对喂食高脂肪/高蔗糖饮食的小鼠的能量代谢、粪便短链脂肪酸产生和微生物群谱的影响。
给3周龄雄性C57BL/6J小鼠喂食含有纤维素或富士FF的高脂肪/高蔗糖饮食12周，并评估其对能量代谢、短链脂肪酸产生和微生物群谱的影响。
与补充纤维素的小鼠相比，在高脂肪/高蔗糖饮食喂养的C57BL/6J小鼠中，补充富士FF可通过减少白色脂肪组织重量同时增加能量消耗来抑制体重增加。富士FF还提高了小鼠粪便中乙酸、丙酸和丁酸的水平，并增加了小鼠血浆丙酸水平。此外，粪便样本的16S rRNA基因扩增子测序显示，与补充纤维素的小鼠相比，补充富士FF的小鼠在门水平上拟杆菌门的丰度升高，厚壁菌门的丰度降低。富士FF还导致粪便中拟杆菌目S24-7科的丰度增加，脱硫弧菌科的丰度降低。
长期食用富士FF可通过改变微生物群组成和增加短链脂肪酸产生来改善小鼠肠道环境，这可能与其抗肥胖作用有关。
