Karlsmark T, Danielsen L, Thomsen H K, Aalund O, Nielsen K G, Nielsen O, Genefke I K
Department of Dermato-venereology and Pathology, Bispebjerg Hospital, Copenhagen, Denmark.
Forensic Sci Int. 1988 Dec;39(3):227-33. doi: 10.1016/0379-0738(88)90125-9.
In order to compare the dermal changes after exposure to direct current (d.c.) with changes after influence of acid and base influence, the skin of fully anaesthetized Danish Landrace pigs were exposed to acid and basic solutions. Biopsies were obtained immediately after and up to day 7 after the injury. Collagen fibres with increased affinity for eosin and irregular cross-striation in polarized light together with shrunken cells with dark stained nuclei were found just beneath the epidermis immediately after application of 1 N HCl. Immediately after exposure to 1 N NaOH dispersed collagen fibres showed increased eosinophilia and a fine densely spaced cross-striation in polarized light and vesicular nuclei were present within dermal cells. During the following days a narrow demarcation zone of neutrophilic granulocytes separated the zone containing abnormal collagen fibres from normal tissue below. Calcified collagen fibres were not observed and no other abnormal histochemical reactions were present. It is concluded that the morphology of acid induced lesions and base induced lesions shows resemblance to the morphology of anode and cathode lesions, respectively, but not to heat lesions. The reason for not finding depositions of calcium salts on collagen fibres in skin exposed to basic solutions could be a non-optimal pH in the tissue or that other electrochemical processes than shift in pH are necessary for the calcification process.
为了比较暴露于直流电（d.c.）后与受到酸和碱影响后的皮肤变化，将完全麻醉的丹麦长白猪的皮肤暴露于酸性和碱性溶液中。在损伤后立即及直至第7天获取活检样本。在涂抹1 N盐酸后，立即在表皮下方发现对伊红亲和力增加且在偏振光下有不规则横纹的胶原纤维，以及细胞核染色深的皱缩细胞。暴露于1 N氢氧化钠后，立即发现分散的胶原纤维嗜伊红性增加，在偏振光下有细密间隔的横纹，且真皮细胞内有泡状核。在接下来的几天里，嗜中性粒细胞形成的狭窄分界区将含有异常胶原纤维的区域与下方的正常组织分隔开来。未观察到钙化的胶原纤维，也未出现其他异常组织化学反应。结论是，酸诱导损伤和碱诱导损伤的形态分别与阳极和阴极损伤的形态相似，但与热损伤不同。在暴露于碱性溶液的皮肤中未在胶原纤维上发现钙盐沉积的原因可能是组织中的pH值不理想，或者钙化过程除了pH值变化外还需要其他电化学过程。
