Karlsmark T, Danielsen L, Thomsen H K, Johnson E, Aalund O, Nielsen K G, Nielsen O, Genefke I K
Department of Dermato-venereology and Pathology, Bispebjerg Hospital, Copenhagen, Denmark.
Forensic Sci Int. 1988 Dec;39(3):235-43. doi: 10.1016/0379-0738(88)90126-0.
In order to describe the ultrastructure of the histopathological changes in dermis after exposure to electrical energy, heat energy and acid and basic solutions the skin of fully anaesthetized Danish Landrace pigs were exposed to direct current, heat (80 degrees C and 450 degrees C) and acid and basic solutions. Biopsies were obtained immediately after the exposure from all types of injury. Biopsies from the cathode areas biopsies were also taken on day 1 and day 2.5 in order to describe the initial calcium deposits. Homogeneous collagen fibres without any birefringence from heat exposed areas were ultrastructurally composed of filamentous materials. Collagen fibres with fine densely spaced cross-striation from cathode areas and areas exposed to basic solutions were shown ultrastructurally to consist of parallelly arranged collagen fibrils with regular waves. It is concluded that the cross-striation of the collagen fibres observed in polarized light are due to a periodic change in the orientation of the fibres seen as waves of the fibres. The ultrastructure of dermal cells were similar to that of epidermal cells following the different types of influence. Characteristically the nuclei were condensed following heat and more electron-lucent following direct current (d.c.) and acid and basic solutions. In cathode areas and areas influenced by basic solutions the electron-lucent nuclei contained fine fibrils. The ultrastructural study supports the suggestion from light microscopic studies that the morphology of anode and cathode lesions shows resemblance to acid induced and basic induced lesions, respectively. Apatite crystals were observed on day 2.5 at the periphery of the collagen fibrils and in the matrix of elastic fibres.
为了描述暴露于电能、热能以及酸碱溶液后真皮组织病理学变化的超微结构，将完全麻醉的丹麦长白猪的皮肤暴露于直流电、热（80摄氏度和450摄氏度）以及酸碱溶液中。在所有类型损伤暴露后立即获取活检组织。还在第1天和第2.5天取阴极区域的活检组织，以描述初始钙沉积情况。热暴露区域中无任何双折射的均匀胶原纤维在超微结构上由丝状物质组成。阴极区域以及暴露于碱性溶液区域中具有细密间隔横纹的胶原纤维在超微结构上显示为由平行排列且有规则波浪状的胶原原纤维组成。得出的结论是，在偏振光下观察到的胶原纤维横纹是由于纤维方向的周期性变化，表现为纤维的波浪状。在不同类型的影响下，真皮细胞的超微结构与表皮细胞相似。其特征在于，热作用后细胞核浓缩，而在直流电、酸和碱性溶液作用后细胞核电子密度更高。在阴极区域以及受碱性溶液影响的区域，电子密度高的细胞核含有细纤维。超微结构研究支持了光镜研究的观点，即阳极和阴极损伤的形态分别与酸诱导和碱诱导的损伤相似。在第2.5天，在胶原原纤维周边和弹性纤维基质中观察到了磷灰石晶体。
