Department of Medicine, Perelman School of Medicine, University of Pennsylvania , Philadelphia, PA, USA.
Platelets. 2020 May 18;31(4):423-431. doi: 10.1080/09537104.2020.1748589. Epub 2020 Apr 16.
A confluence of technological advances in genetic manipulation and molecular-based fluorescence imaging has led to the widespread adoption of laser injury models to study hemostasis and thrombosis in mice. In all animal models of hemostasis and thrombosis, detailing the nature of experimentally induced vascular injury is paramount in enabling appropriate interpretation of experimental results. A careful appraisal of the literature shows that direct laser-induced injury can result in variable degrees of vascular damage. This review will compare and contrast models of laser injury utilized in the field, with an emphasis on the mechanism and extent of injury, the use of laser injury in different vascular beds and the molecular mechanisms regulating the response to injury. All of these topics will be discussed in the context of how distinct applications of laser injury models may be viewed as representing thrombosis and/or hemostasis.
遗传操作和基于分子的荧光成像技术的进步使得激光损伤模型在研究止血和血栓形成方面得到了广泛应用。在所有止血和血栓形成的动物模型中，详细描述实验性诱导的血管损伤的性质对于正确解释实验结果至关重要。仔细研究文献表明，直接激光诱导损伤可导致不同程度的血管损伤。本文综述比较和对比了激光损伤模型在该领域的应用，重点介绍损伤的机制和程度、激光损伤在不同血管床的应用以及调节损伤反应的分子机制。所有这些主题都将在激光损伤模型的不同应用如何被视为代表血栓形成和/或止血的背景下进行讨论。
