Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Chem Commun (Camb). 2020 May 27;56(42):5617-5620. doi: 10.1039/d0cc00559b.
We show that the equilibrium of intramolecular spirocyclization of coumarin-hemicyanine hybrid fluorophores can be finely tuned by means of chemical modifications. We used this scaffold to develop activatable fluorescent probes with large Stokes shifts for γ-glutamyltranspeptidase and esterase.
我们表明，通过化学修饰可以精细调节香豆素-半花菁杂化荧光团分子内环化平衡。我们利用这个支架开发了用于γ-谷氨酰转肽酶和酯酶的具有大斯托克斯位移的活化荧光探针。
