基于β-咔啉的 pH 荧光探针及其用于监测酶促酯水解的应用。

β-Carboline-Based pH Fluorescent Probe and Its Application for Monitoring Enzymatic Ester Hydrolysis.

机构信息

School of Pharmaceutical Sciences, South-Central University of Nationalities, Wuhan, 430074, P. R. China.

National Demonstration Center for Experimental Ethnopharmacology Education, South-Central University of Nationalities, Wuhan, 430074, P. R. China.

出版信息

Chem Biodivers. 2021 Jan;18(1):e2000829. doi: 10.1002/cbdv.202000829. Epub 2020 Dec 3.

DOI:10.1002/cbdv.202000829

PMID:33188535

Abstract

A novel pH-activatable fluorescent probe, 1-(propan-2-yl)-9H-pyrido[3,4-b]indole-3-carboxylic acid (L-1), based on β-carboline derivatives, has been developed, which displays significant fluorescent response toward pH variation with high selectivity, good photo-stability and favorable pKa value. Moreover, L-1 can dynamically monitor the release of protons during ester hydrolysis reaction in consistent with enzymatic kinetics manner.

摘要

一种新型的 pH 激活荧光探针，1-(异丙基)-9H-吡啶并[3,4-b]吲哚-3-羧酸（L-1），基于β-咔啉衍生物，已被开发出来，它对 pH 值的变化具有显著的荧光响应，具有高选择性、良好的光稳定性和合适的 pKa 值。此外，L-1 可以在酯水解反应过程中动态监测质子的释放，与酶动力学方式一致。