Department of Ophthalmology, University of Würzburg, Würzburg, Germany.
Department of Ophthalmology, Xiangya Hospital, Central South University, Changsha, Hunan, China.
PLoS One. 2020 Apr 16;15(4):e0231360. doi: 10.1371/journal.pone.0231360. eCollection 2020.
To investigate whether microsurgical excision of trabecular meshwork (TM) in an ex vivo pigmentary glaucoma model can normalize the hypertensive phenotype.
Eight eyes of a porcine pigmentary glaucoma model underwent 90° of microsurgical TM excision with an aspirating dual-blade (Goniotome (G)). 24 hours later, additional 90° of TM were removed. Anterior segments with sham surgeries served as the control (C). Outflow facility and intraocular pressure (IOP) were analyzed. Histology with hematoxylin and eosin (H&E) was obtained.
After the first 90° TM excision, IOP was significantly lower in G (10.2±2.4 mmHg, n = 7) than C (20.0±2.0mmHg, n = 8, P<0.01). Outflow facility in G (0.38±0.07 μl/min/mmHg) was higher than C (0.16±0.02 μl/min/mmHg, P<0.01). After the second 90° TM excision, IOP in G (6.46±0.81 mmHg, n = 7) was significantly lower than C (20.3±1.7 mmHg, n = 8, P<0.001), while the outflow facility in G (0.50±0.05 μl/min/mmHg, n = 7) was higher than C (0.16±0.01 μl/min/mmHg, n = 8, P<0.001). Compared to the first excision, excision of an additional 90° did not change of IOP (P = 0.20) or outflow facility (P = 0.17) further.
Excision of 90° of TM in a pigmentary glaucoma model using an aspirating dual-blade decreased IOP and increased outflow facility.
研究在体外色素性青光眼模型中是否可以通过小梁网（TM）显微切除术使高血压表型正常化。
猪色素性青光眼模型中的 8 只眼接受了 90°的抽吸式双刀片（Goniotome（G））TM 切除术。24 小时后，再切除 90°的 TM。接受假手术的前段作为对照（C）。进行房水流出率和眼内压（IOP）分析。用苏木精和伊红（H＆E）进行组织学检查。
第一次切除 90°TM 后，G（10.2±2.4mmHg，n=7）的 IOP 明显低于 C（20.0±2.0mmHg，n=8，P<0.01）。G 的房水流出率（0.38±0.07μl/min/mmHg）高于 C（0.16±0.02μl/min/mmHg，P<0.01）。第二次切除 90°TM 后，G 的 IOP（6.46±0.81mmHg，n=7）明显低于 C（20.3±1.7mmHg，n=8，P<0.001），而 G 的房水流出率（0.50±0.05μl/min/mmHg，n=7）高于 C（0.16±0.01μl/min/mmHg，n=8，P<0.001）。与第一次切除相比，进一步切除 90°TM 并未改变眼压（P=0.20）或房水流出率（P=0.17）。
在色素性青光眼模型中使用抽吸式双刀片切除 90°TM 可降低眼压并增加房水流出率。
