Haye-Legrand I, Norel X, Labat C, Benveniste J, Brink C
INSERM U.200, Université Paris Sud, Clamart, France.
Int Arch Allergy Appl Immunol. 1988;87(4):342-8. doi: 10.1159/000234699.
Spirally cut guinea pig tracheal preparations were passively sensitized using a mouse monoclonal IgE antibody against dinitrophenol (DNP). Maximal contraction observed following challenge with DNP-bovine serum albumin (DNP-BSA, 5 micrograms/ml; n = 20) response was approximately 46% of the histamine response (0.52 +/- 0.09 g/mm2; n = 53). Indomethacin (1.7 microM) increased and PGE2 (1 microM) decreased the response to the antigen. FPL 55712 (10 microM), atropine (0.1 microM), L 651392 (5 microM) or tripelennamine (1 microM) always reduced the maximal DNP-BSA response, but not BN 52021 (100 microM). This model may be used for rapid detection of compounds with antiallergic properties on IgE-dependent lung pathological states.
使用抗二硝基苯酚（DNP）的小鼠单克隆IgE抗体对螺旋切割的豚鼠气管制剂进行被动致敏。用DNP-牛血清白蛋白（DNP-BSA，5微克/毫升；n = 20）激发后观察到的最大收缩反应约为组胺反应（0.52±0.09克/平方毫米；n = 53）的46%。吲哚美辛（1.7微摩尔）增强而前列腺素E2（1微摩尔）减弱对抗原的反应。FPL 55712（10微摩尔）、阿托品（0.1微摩尔）、L 651392（5微摩尔）或曲普利啶（1微摩尔）总是降低最大DNP-BSA反应，但BN 52021（100微摩尔）则不然。该模型可用于快速检测对IgE依赖性肺部病理状态具有抗过敏特性的化合物。
