Pirotzky E, Pintos-Morell G, Burtin C, Boudet J, Bidault J, Benveniste J
INSERM U.200, Université Paris-Sud, Clamart, France.
Kidney Int. 1987 Aug;32(2):233-7. doi: 10.1038/ki.1987.197.
Isolated perfused rat kidneys were passively sensitized by addition of either mouse ascitic fluid containing monoclonal IgE against dinitrophenol (DNP) or DNP-specific purified IgE. After washing the organ, defined doses of DNP-bovine serum albumin were given as bolus injection via the kidney artery. Antigen challenge of IgE-sensitized kidneys resulted in a dose-dependent increase of perfusion pressure starting with 5 micrograms antigen (2.46 +/- 0.2 mm Hg) and reaching a maximum at dose higher than 100 micrograms (10.3 +/- 1.6 mm Hg) (N = 4, means +/- 1 SD). A decrease of glomerular filtration rate was also observed which reached a plateau at 100 micrograms antigen (-68.5 +/- 2.9%) (N = 4). Regardless of the dose of antigen used, the urinary protein excretion markedly increased for the first five minutes following antigen injection and returned to basal values after 10 minutes. The total amounts of histamine, PGE2 and paf-acether (platelet-activating factor) released upon antigen challenge (1 mg) for 15 minutes reached maximal values of 405 +/- 21.1 ng, 286 +/- 19.4 pg and 12.3 +/- 3.2 ng (N = 5), respectively. None of these hemodynamic and biochemical effects were observed using IgG1 monoclonal antibodies or when the ascitic fluid containing monoclonal IgE used to sensitize the organ was heated at 56 degrees C for two hours. Thus, we have described a pure IgE-dependent rat kidney anaphylaxis. Antigen challenge markedly altered renal parameters and triggered the release of various mediators from the organ, suggesting that type I-hypersensitivity reactions may play a role in renal pathophysiology.
通过添加含有抗二硝基苯酚（DNP）的单克隆IgE的小鼠腹水或DNP特异性纯化IgE，对分离的灌注大鼠肾脏进行被动致敏。冲洗器官后，通过肾动脉推注给予确定剂量的DNP-牛血清白蛋白。用IgE致敏的肾脏进行抗原攻击导致灌注压呈剂量依赖性增加，从5微克抗原开始（2.46±0.2毫米汞柱），在高于100微克的剂量时达到最大值（10.3±1.6毫米汞柱）（N = 4，平均值±1标准差）。还观察到肾小球滤过率下降，在100微克抗原时达到平台期（-68.5±2.9%）（N = 4）。无论使用的抗原剂量如何，抗原注射后的前五分钟尿蛋白排泄量显著增加，10分钟后恢复到基础值。抗原攻击（1毫克）15分钟后释放的组胺、PGE2和血小板激活因子（PAF）的总量分别达到最大值405±21.1纳克、286±19.4皮克和12.3±3.2纳克（N = 5）。使用IgG1单克隆抗体或用于致敏器官的含有单克隆IgE的腹水在56℃加热两小时时，未观察到这些血流动力学和生化效应。因此，我们描述了一种纯IgE依赖性大鼠肾脏过敏反应。抗原攻击显著改变了肾脏参数并触发了器官释放各种介质，表明I型超敏反应可能在肾脏病理生理学中起作用。
