School of Nursing, Xuzhou Medical University, Xuzhou, China.
Department of Anesthesiology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, 221002, China.
Neurosci Lett. 2020 May 29;728:134972. doi: 10.1016/j.neulet.2020.134972. Epub 2020 Apr 13.
The cerebrospinal fluid (CSF)-contacting nucleus is a special nucleus. To study the mechanism of the CSF-contacting nucleus in learning and memory, we used classic retrograde tracing methods to observe the synaptic connections between the CSF-contacting nucleus and the hippocampus. By injecting cholera toxin B subunit (CB) - saporin (SAP) into the lateral ventricle of animals to exclusively damage this nucleus, a mature CSF-contacting nucleus-deficient model animal was established. Then, the changes in learning and memory behaviors in animals with "damage" or "compensation" after damage to the CSF-contacting nucleus were studied. The results showed that learning and memory abilities in animals decreased significantly after the destruction of the CSF-contacting nucleus, accompanied by a decrease in 5-HT concentrations in hippocampus. However, after compensating for 5-HT in the hippocampus continuously, the learning and memory abilities of the animals were significantly improved. This study suggests that the CSF-contacting nucleus may participate in the regulation of learning and memory through direct synaptic connections with the hippocampus.
脑脊髓液接触核是一种特殊的核。为了研究脑脊髓液接触核在学习和记忆中的机制，我们使用经典的逆行追踪方法观察了脑脊髓液接触核与海马之间的突触连接。通过将霍乱毒素 B 亚单位（CB）-SAP 注入动物的侧脑室，专门破坏这个核，建立了一个成熟的脑脊髓液接触核缺失模型动物。然后，研究了脑脊髓液接触核损伤后动物的“损伤”或“补偿”后的学习和记忆行为变化。结果表明，脑脊髓液接触核破坏后，动物的学习和记忆能力显著下降，同时海马体中 5-HT 浓度降低。然而，在持续补偿海马体中的 5-HT 后，动物的学习和记忆能力显著提高。这项研究表明，脑脊髓液接触核可能通过与海马体的直接突触连接参与学习和记忆的调节。
