Song Si-Yuan, Zhang Li-Cai
Jiangsu Province Key Laboratory of Anesthesiology, Xuzhou Medical University, Xuzhou, China.
Front Neuroanat. 2018 Mar 27;12:22. doi: 10.3389/fnana.2018.00022. eCollection 2018.
To establish an entirely cerebrospinal fluid (CSF)-contacting nucleus-deficient model animal, we used cholera toxin B subunit (CB)- saporin (SAP), which is an analog of CB-HRP that specifically labels the CSF-contacting nucleus, to exclusively damage the nucleus. The effectiveness and specificity of the ablation were evaluated upon days 1-10 after CB-SAP microinjection into the brain ventricular system. The vital status, survival, and common physiological parameters of the model animals were also assessed during the experimental period. The results demonstrated that CB-SAP damaged only the CSF-contacting nucleus, but not other functional structures, in the brain. The complete ablation occurred by day 7 after CB-SAP microinjection. A model animal that had no CSF-contacting nucleus was established after survival beyond that time point. No obvious effects were observed in the vital status of the model animals, and their survival was ensured. The common physiological parameters of model animals were stable. The present study provides a method to establish a CSF-contacting nucleus "knockout" model animal, which is similar to a gene knockout model animal for studying this particular nucleus .
为建立一个完全缺乏脑脊液（CSF）接触核的模型动物，我们使用了霍乱毒素B亚基（CB）-皂草素（SAP），它是CB-辣根过氧化物酶（HRP）的类似物，能特异性标记CSF接触核，以专门损伤该核。在将CB-SAP微量注射到脑室系统后的第1至10天，评估损毁的有效性和特异性。在实验期间，还评估了模型动物的生命状态、存活情况和常见生理参数。结果表明，CB-SAP仅损伤了脑中的CSF接触核，而未损伤其他功能结构。在CB-SAP微量注射后第7天实现了完全损毁。在存活超过该时间点后，建立了没有CSF接触核的模型动物。未观察到模型动物生命状态有明显影响，且确保了它们的存活。模型动物的常见生理参数稳定。本研究提供了一种建立CSF接触核“敲除”模型动物的方法，这类似于用于研究该特定核的基因敲除模型动物。
