Ostróżka-Cieślik Aneta, Dolińska Barbara, Ryszka Florian
Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, Katowice, Poland.
Biochefa Pharmaceutical Research and Production Plant, Sosnowiec, Poland.
Transplant Proc. 2020 Sep;52(7):2055-2058. doi: 10.1016/j.transproceed.2020.01.100. Epub 2020 Apr 14.
Lutropin, a luteinizing hormone (LH), is a glycoprotein hormone secreted by gonadotropic cells of the anterior pituitary gland. LH receptors are a promising therapeutic target. The aim of the study was to analyze the protective effect of LH as a component of preservation solutions on the prevention of ischemia-reperfusion injury in isolated porcine kidneys.
The study used Biolasol solution (Biochefa, Sosnowiec, Poland) modified with the addition of ascorbic acid and LH at a concentration of 0.01 μg/L, 0.1 μg/L, and 1 μg/L. The study was carried out on 40 kidneys from 20 Polish large white pigs. Kidneys were divided into 4 groups, then washed out of the blood, and cooled by complete immersion in a 500-mL preservation solution at 4°C. The total storage time for grafts in the preservation solutions was 2 hours. After this time, the kidneys were perfused. Perfundates intended for biochemical analyses were collected from the renal vein after 0 minutes and after 30 minutes of perfusion. Another perfusion was performed after 24-hour renal storage. The activity of aspartate aminotransferase and alanine aminotransferase, as well as sodium, potassium, total protein, and urea concentrations was determined.
LH as a component of preservation solutions affects the degree of ischemia-reperfusion injury of isolated porcine kidneys.
The results obtained suggest that the efficacy of LH in improving ischemic kidney function is dependent on the dose used. The best graft protection was obtained using the lowest concentration of LH: 0.01 μg/L.
促黄体激素（LH）是一种促性腺激素，由腺垂体促性腺细胞分泌的糖蛋白激素。LH受体是一个有前景的治疗靶点。本研究的目的是分析LH作为保存液成分对预防离体猪肾缺血再灌注损伤的保护作用。
本研究使用添加了浓度为0.01μg/L、0.1μg/L和1μg/L的抗坏血酸和LH的Biolosol溶液（Biochefa，索斯诺维茨，波兰）。研究对来自20头波兰大白猪的40个肾脏进行。将肾脏分为4组，然后冲洗出血液，并通过完全浸入4℃的500mL保存液中进行冷却。移植物在保存液中的总保存时间为2小时。此后，对肾脏进行灌注。在灌注0分钟和30分钟后从肾静脉收集用于生化分析的灌注液。在肾脏保存24小时后进行另一次灌注。测定天冬氨酸转氨酶和丙氨酸转氨酶的活性以及钠、钾、总蛋白和尿素的浓度。
LH作为保存液的成分影响离体猪肾缺血再灌注损伤的程度。
所得结果表明，LH改善缺血肾功能的疗效取决于所用剂量。使用最低浓度的LH：0.01μg/L可获得最佳的移植物保护效果。
