Ryszka F, Dolińska B, Czyż K, Jelińska M, Strabel A, Bocheńska J
Pharmaceutical Research and Production Plant "Biochefa", Sosnowiec, Poland.
Pharmaceutical Research and Production Plant "Biochefa", Sosnowiec, Poland; Department of Applied Pharmacy, Medical University of Silesia, Sosnowiec, Poland.
Transplant Proc. 2016 Jun;48(5):1824-8. doi: 10.1016/j.transproceed.2015.12.140.
The influence of recombinant human prolactin (rh-PRL) added to Biolasol solution (concentration 1 μg/L) on selected markers (pH, osmolarity, Na(I) and K(I) concentration) and enzymatic activity (alanine aminotransferase [ALT], aspartate aminotransferase [AST], and lactate dehydrogenase [LDH]) in perfundates was investigated during flushing and preservation of the isolated porcine kidneys.
The pH, osmolarity, concentration of K(I) and Na(I), and enzymatic activity were determined in perfundates collected after the 5th and 30th minutes of perfusion, after 24 hours of organ preservation, and in the 5th and 30th minutes of reperfusion. Kidneys had been flushed and stored in Biolasol (control group) and in Biolasol with rh-PRL (experimental group). Obtained results were compared with Biolasol solution.
In the experimental group, the decrease in pH value in the 5th minute of reperfusion was noted. There was an increase in K(I) concentration, and Na(I) concentration decreased in the 5th and 30th minutes of reperfusion. ALT activity during perfusion and preservation increased, whereas at the 5th and 30th minutes of reperfusion it decreased. AST activity increased during perfusion and preservation and decreased in the 5th and 30th minutes of reperfusion. LDH activity was increased but decreased in the 5th minute of reperfusion.
Addition of 1 μg/L rh-PRL to Biolasol solution decreases pH and osmolarity values; influences Na(I) and K(I) concentration; increases ALT, AST activity during perfusion and preservation of organs; and decreases ALT, AST activity during reperfusion.
在离体猪肾冲洗和保存过程中，研究了添加到Biolasol溶液（浓度为1μg/L）中的重组人催乳素（rh-PRL）对灌注液中选定指标（pH值、渗透压、钠和钾浓度）以及酶活性（丙氨酸转氨酶[ALT]、天冬氨酸转氨酶[AST]和乳酸脱氢酶[LDH]）的影响。
在灌注第5分钟和第30分钟后、器官保存24小时后以及再灌注第5分钟和第30分钟时，测定收集的灌注液中的pH值、渗透压、钾和钠浓度以及酶活性。肾脏分别用Biolasol（对照组）和含rh-PRL的Biolasol（实验组）进行冲洗和保存。将所得结果与Biolasol溶液进行比较。
在实验组中，观察到再灌注第5分钟时pH值下降。再灌注第5分钟和第30分钟时，钾浓度升高，钠浓度降低。灌注和保存期间ALT活性升高，而在再灌注第5分钟和第30分钟时降低。AST活性在灌注和保存期间升高，在再灌注第5分钟和第30分钟时降低。LDH活性升高，但在再灌注第5分钟时降低。
向Biolasol溶液中添加1μg/L rh-PRL可降低pH值和渗透压；影响钠和钾浓度；在器官灌注和保存期间增加ALT、AST活性；并在再灌注期间降低ALT、AST活性。
