Immunohistochemical investigation on the hog kidney di-isopropyl-fluorophosphate fluorohydrolase (E.C.3.8.2.1) in hog kidney and heart.

Using a polyclonal antibody raised in rabbits against the highly purified di-isopropyl-fluorophosphate fluorohydrolase (DFPase, E.C. 3.8.2.1) of hog kidney, DFPase-immunoreactivity could be demonstrated by the unlabelled antibody peroxidase-antiperoxidase immunohistochemical method in the hog kidney in the brush border of epithelial cells in the proximal segment of the nephron. In the heart DFPase-immunoreactivity was found at the plasma membranes of the cardiac muscle cells.