Shiozawa M, Yamashita S, Aiso S, Yasuda K
Department of Anatomy, School of Medicine, Keio University, Tokyo, Japan.
J Histochem Cytochem. 1989 Jul;37(7):1053-61. doi: 10.1177/37.7.2567299.
To perform immunohistochemical study of the distribution of gamma-glutamyl transpeptidase in human organs, a highly specific antibody against the human enzyme is required. We prepared monoclonal antibody against gamma-glutamyl transpeptidase from human kidney, using the hybridoma technique. The antibody was of the IgG1 type and the light chain belonged to the kappa class. The antibody reacted specifically with the 63 KD heavy subunit of the enzyme. Examination of the specificity of the antibody performed by immunohistochemical staining of human kidney sections revealed that the antigen was localized on the brush border and along the basolateral membrane of the epithelial cells of both the convoluted and the straight portions of the proximal tubule. This antibody was also reactive in several human organs other than kidney, including epididymis, prostate, seminal vesicle, pancreas, and normal liver, and in human hepatoma. These findings indicate the existence of an antigenic determinant common to human kidney and other organs. The monoclonal antibody did not crossreact with mouse, rat, guinea pig, rabbit, or pig kidney.
为了对人器官中γ-谷氨酰转肽酶的分布进行免疫组织化学研究，需要一种针对该人类酶的高度特异性抗体。我们采用杂交瘤技术，制备了针对人肾γ-谷氨酰转肽酶的单克隆抗体。该抗体为IgG1型，轻链属于κ类。该抗体与该酶的63KD重亚基发生特异性反应。通过对人肾切片进行免疫组织化学染色来检测抗体的特异性，结果显示抗原定位于近端小管曲部和直部上皮细胞的刷状缘以及基底外侧膜上。该抗体在除肾脏外的其他几个人类器官中也有反应，包括附睾、前列腺、精囊、胰腺和正常肝脏，以及人类肝癌组织。这些发现表明人肾和其他器官存在共同的抗原决定簇。该单克隆抗体与小鼠、大鼠、豚鼠、兔子或猪的肾脏不发生交叉反应。
