Barletta Mário, Costa Monica F, Dantas David V
Laboratório de Ecologia e Gerenciamento de Ecossistemas Costeiros e Estuarinos (LEGECE), Departamento de Oceanografia (DOCEAN), Universidade Federal de Pernambuco (UFPE), Campus Universitário, Recife, Pernambuco 50740-550, Brazil.
Programa de Pós-graduação em Planejamento Territorial e Desenvolvimento Socioambiental (PPGPLAN/FAED), Grupo de Gestão, Ecologia e Tecnologia Marinha (GTMar), Departamento de Engenharia de Pesca e Ciências Biológicas (DEPB), Universidade do Estado de Santa Catarina (UDESC), Laguna, Santa Catarina, Brazil.
MethodsX. 2020 Mar 19;7:100861. doi: 10.1016/j.mex.2020.100861. eCollection 2020.
The aim was to describe a methodology developed to study the relationship among the spatio-temporal patterns of habitat utilization, feeding ecology and microplastics (MPs) contamination across the different ontogenetic phases of fishes belonging to different trophic levels and living along the riverine-estuarine-coastal food web. The Goiana Estuary's water column was examined for the seasonal and spatial variation of MPs and their quantification relative to zooplankton, demersal fish species contamination following the same sampling design. The density of MPs in the water column determines their bioavailability. Interest in studies on MPs distribution in relation to spatial and temporal variation of environmental factors and fauna are increasing in quantity and quality. If the ecological strategies presented in this study were replicated in other estuary, comparisons could be made in order to describe how ecosystems work. Standard protocols for sampling, extraction, enumeration and classification of MPs and others pollutant ingested by fishes have been developed and are presented here to encourage comparisons. Standardized and comparable sampling designs and laboratory procedures are an important strategy in order to devise and transfer managerial solutions among different sites and comparisons along time when studying the same environment.
目的是描述一种所开发的方法，用于研究不同营养级且生活在河川 - 河口 - 沿海食物网中的鱼类在不同个体发育阶段的栖息地利用时空模式、摄食生态学与微塑料（MPs）污染之间的关系。按照相同的采样设计，对戈亚尼亚河口的水柱进行了微塑料的季节和空间变化及其相对于浮游动物的量化研究，以及底栖鱼类物种的污染情况研究。水柱中微塑料的密度决定了它们的生物可利用性。关于微塑料分布与环境因素和动物群时空变化关系的研究在数量和质量上都在增加。如果本研究中提出的生态策略能在其他河口得到复制，就可以进行比较，以描述生态系统是如何运作的。本文制定并展示了用于鱼类摄入的微塑料及其他污染物的采样、提取、计数和分类的标准方案，以鼓励进行比较。标准化且可比的采样设计和实验室程序是一项重要策略，以便在研究同一环境时，在不同地点之间以及随时间推移设计并转移管理解决方案。
